Category:ECO:0005598 ! wide-field fluorescence microscopy evidence

name: wide-field fluorescence microscopy evidence
def: "A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." [ECO:SN, url:http\://www.bristol.ac.uk/synaptic/research/techniques/widefield.html]
is_a: ECO:0005588 ! wide-field microscopy evidence
comment: The optical filter is used to select the wavelength of excitation light. The detection camera is typically a CCD camera. Both topographical and dynamic information of a sample are obtained.