Category:ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence

name: two-dimensional polyacrylamide gel electrophoresis evidence
def: "A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape." [ECO:SW, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html]
synonym: "2D PAGE" EXACT []
synonym: "2D-PAGE" EXACT []
is_a: ECO:0000337 ! gel electrophoresis evidence
comment: Two-dimensional polyacrylamide gel electrophoresis is commonly employed to establish a proteome for an organism and demonstrate expression changes.