id: ECO:0001824
name: DNA adenine methyltransferase identification evidence
def: "A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site." [ECO:SW, PMID:16938559, PMID:17545983, PMID:19588092]
synonym: "DamID" EXACT [PMID:19588092]
is_a: ECO:0000023 ! affinity evidence
is_a: IAO:0000030 ! information content entity
relationship: IAO:0000136: GO:0008104 ! is about protein localization
comment: The methylation tag is detected with methylation-sensitive restriction enzymes, DpnI and DpnII.
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Last version checked
releases/2019-06-14.
14:06:2019 08:25.
rctauber | Last updated
releases/2019-06-14 | |
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