id: ECO:0001819
name: primer extension assay evidence
def: "A type of transcript expression evidence used to determine expression\nlevels of mRNA where a labeled synthetic oligonucleotide primer is annealed to mRNA downstream of the presumed transcription start site of a gene. The mRNA is extended with reverse transcriptase, producing cDNA which is electrophoresed, and the band size is enables determination of the 5' end of the mRNA (i.e. the transcription start site)." [ECO:SW, PMID:23378648]
is_a: ECO:0000009 ! transcript expression evidence
comment: The most commonly used radiolabel is 32P.
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Last version checked
releases/2019-06-14.
14:06:2019 08:25.
rctauber | Last updated
releases/2018-12-17 | |
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