Category:ECO:0000339 ! two-dimensional agarose gel electrophoresis evidence

name: two-dimensional agarose gel electrophoresis evidence
def: "A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide." [PMID:16118435, PMID:8594382]
synonym: "2-D agarose gel electrophoresis" EXACT []
is_a: ECO:0000337 ! gel electrophoresis evidence
comment: The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape.