id: ECO:0000333
name: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
def: "A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility." [ECO:MCC, PubMed:4861258, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html, url:http\://www.ncbi.nlm.nih.gov/Class/NAWBIS/Modules/Protein/protein18.html]
synonym: "SDS-PAGE evidence" EXACT []
is_a: ECO:0000337 ! gel electrophoresis evidence
comment: Electrophoretic mobility is a function of both the length and charge of a protein. The protein sample is linearized with sodium dodecyl sulfate (SDS), an anionic detergent. Typically, polypeptides bound with SDS possess an even distribution of charge per unit mass, and subsequent fractionation is by size.
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Last version checked
releases/2019-06-14.
14:06:2019 08:25.
rctauber | Last updated
releases/2015-12-04 | |
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