Category:ECO:0000226 ! chromatin immunoprecipitation evidence

name: chromatin immunoprecipitation evidence
def: "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]
synonym: "ChIP evidence" EXACT []
xref: [ MI:0402 "chromatin immunoprecipitation assay"]
is_a: ECO:0000085 ! immunoprecipitation evidence
relationship: IAO:0000136: GO:0043565 ! is about sequence-specific DNA binding
relationship: OBI:0000312: OBI:0001956 ! is_specified_output_of assay using chromatin immunoprecipitation
comment: Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).