Category:ECO:0000118 ! subtractive hybridization evidence

name: subtractive hybridization evidence
def: "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'." [PMID:11298187]
synonym: "IEP: subtractive hybridization" RELATED []
is_a: ECO:0000009 ! transcript expression evidence
relationship: IAO:0000136: GO:0010467 ! is about gene expression
relationship: OBI:0000312: OBI:0000424 ! is_specified_output_of transcription profiling assay
comment: Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries.