Category:ECO:0000112 ! western blot evidence

name: western blot evidence
def: "A type of gel electrophoresis evidence where proteins are separated using gel electrophoresis and then they are blotted on a nitrocellulose or polyvinylidene difluoride (PVDF) membrane, later an antibody with a dye or an enzyme is added to the solution which is able to bind to its specific protein and this product is detected either by chemiluminescent detection technique or fluorescence imaging technique." [ECO:SW, PMID:23050259, TAIR:TED]
synonym: "IEP: protein levels (e.g. Western blots)" RELATED []
synonym: "protein immunoblot" RELATED []
synonym: "Western blot expression analysis" RELATED []
is_a: ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
is_a: ECO:0005593 ! immunodetection assay evidence
comment: Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies.