id: ECO:0000109
name: reverse transcription polymerase chain reaction evidence
def: "A type of RNA detection assay evidence where an RNA transcript is reverse transcribed into cDNA and amplified to qualitatively detect gene expression." [ECO:SW, PMID:11013345, PMID:12901609]
alt_id: ECO:0000108
synonym: "IEP: transcript levels (e.g. RT-PCR)" RELATED []
synonym: "reverse transcription polymerase chain reaction transcription evidence" BROAD []
synonym: "RT-PCR" EXACT []
is_a: ECO:0005518 ! RNA detection assay evidence
relationship: IAO:0000136: GO:0010467 ! is about gene expression
relationship: OBI:0000312: OBI:0001361 ! is_specified_output_of transcription profiling by RT-PCR assay
comment: The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate.
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Last version checked
releases/2019-06-14.
14:06:2019 08:25.
rctauber | Last updated
releases/2019-06-14 | |
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Pages in category "ECO:0000109 ! reverse transcription polymerase chain reaction evidence"
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