Category:ECO:0000086 ! amplification of intermethylated sites evidence

name: amplification of intermethylated sites evidence
def: "A type of in vitro methylation assay evidence resulting from a genome-wide estimate of DNA methylation by differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers, followed by restrained PCR amplification of sequences methylated at both ends and resolving the PCR products in a denaturing polyacrylamide-sequencing gel to generate fingerprints that consist of multiple anonymous bands that represent the DNA methylome of the cell." [PMID:18987810, url:https\://www.ncbi.nlm.nih.gov/probe/docs/techpcr/]
synonym: "AIMS" EXACT []
is_a: ECO:0001065 ! in vitro methylation assay evidence
relationship: IAO:0000136: GO:0006306 ! is about DNA methylation
relationship: OBI:0000312: OBI:0001685 ! is_specified_output_of amplification of intermethylated sites assay
comment: The bands can be individually isolated and characterized which leads to the identification of hypo- and hypermethylation events.