Category:ECO:0000069 ! differential methylation hybridization evidence

name: differential methylation hybridization evidence
def: "A type of in vitro methylation assay evidence where methylation-sensitive restriction enzymes are utilized to compare differential methylation between two samples by first shearing DNA, followed by end-blunting, ligation of linkers, methylation sensitive restriction, PCR using linker primers, dye labeling and relative quantification of methylated DNA fragments by two-colored array hybridization to a CpG island microarray for visual assessment." [PMID:18987809]
alt_id: ECO:0000065
synonym: "CpG island microarray evidence" EXACT []
is_a: ECO:0001065 ! in vitro methylation assay evidence
relationship: IAO:0000136: GO:0044030 ! is about regulation of DNA methylation
relationship: OBI:0000312: OBI:0001861 ! is_specified_output_of methylation-sensitive restriction enzyme sequencing assay
comment: Color is indicative of methylation status - be it hypo- (pseudo-green), hyper- (pseudo-red), or equal methylation (pseudo-yellow).