Category:5-Bromouracil Mutagenesis
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Purpose
- DNA mutagenesis[1]
Materials Needed
Protocol
Links
User notes
- 2 kinds of mutagenic action leads to 5BU-directed base-pair transitions
- errors of incorporation = rare substitutions of 5BU for cytosine (results in the formation of an AT pair instead of a GC pair)
- errors of replication = 5BU pairs with guanine instead of adenine (result in substitution of a GC pair instead of an AT pair)
References
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- ↑ WITKIN, EM & SICURELLA, NA (1964) PURE CLONES OF LACTOSE-NEGATIVE MUTANTS OBTAINED IN ESCHERICHIA COLI AFTER TREATMENT WITH 5-BROMOURACIL. J. Mol. Biol. 8 610-3 PubMed OMPwiki page
Pages in category "5-Bromouracil Mutagenesis"
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