PMID:5330222
From OMPwiki
| Citation |
Müller-Hill, B (1966) Suppressible regulator constitutive mutants of the lactose system in Escherichia coli.J. Mol. Biol. 15:374-6 |
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| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Enzyme Repression; Escherichia coli; Galactosidases; Genetic Code; Guanidines; Lactose; Molecular Biology; Mutation; Purines; RNA, Messenger |
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Main Points of the Paper
- Suppressors of nonsense codons exist for the regulatory i gene of the lactose operon, thereby proving that the functional i product is a protein.
- Rationale: suppression of nonsense codons into amino acids occurs at the translational level
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- Mutagenesis of E.coli strain 112-12 (gal-, cys-, his-, su)
- Mutagen-producing agents: 2-aminopurine or N-methyl-N'-nitro-N-nitrosguanidine
- ONPG solution to select for lac constitutive mutants
Phenotype Annotations
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
112-12lacc |
lacI |
lac constitutive |
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