OMP ST:1123 ! Escherichia coli K-12 JW3132

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Strain Summary

Strain Name

Escherichia coli K-12 JW3132

Synonyms

JW3132-1

Taxon Information
  • Pangenome: Escherichia coli
  • Subspecies and/or strain: K-12
  • NCBI Taxonomy ID: 83333
Genotype

DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 nlpI775(del)::kan

Strain Reference

Coli Genetic Stock Center

Strain availability

Coli Genetic Stock Center

Ancestry

parent:OMP ST:800 ! Escherichia coli K-12 BW25113

Annotated phenotypes


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Notes

Phenotype Annotation

Annotation ID Qualifier OMP ID OMP term name Relative phenotype information Experimental condition ECO ID ECO term name Reference Annotation Extension Notes

OMP_AN:1039

OMP:0007092

decreased cell motility by swarming

Relative to: OMP_AN:881

Genotype differences:

+DE(araD-araB)567 +DE(rhaD-rhaB)568 +hsdR514 +lacZ4787(del)( +nlpI775(del) -IN(rrnD-rrnE)1

Condition differences:

no differences

  • temperature:33C
  • medium:swarming motility medium
 ECO:0005803

motility assay evidence

PMID:17122336[1]

OMP_AN:33015

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27575

Genotype differences:

+lacZ4787(del)( +nlpI775(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Acriflavine (acriflavinium chloride)
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:74728) Acriflavine (acriflavinium chloride)

  • chemical concentration= 2 ug/ml
  • normalized fitness= -4.45

OMP_AN:33016

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27552

Genotype differences:

+lacZ4787(del)( +nlpI775(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azidothymidine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:10110) Azidothymidine

  • chemical concentration= 2.5 ng/ml
  • normalized fitness= -4.96

OMP_AN:33017

OMP:0006036

decreased resistance to macrolide

Relative to: OMP_AN:27518

Genotype differences:

+lacZ4787(del)( +nlpI775(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Azithromycin
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:2955) Azithromycin

  • chemical concentration= 1 ug/ml
  • normalized fitness= -5.79

OMP_AN:33018

OMP:0007249

decreased resistance to a chemical

Relative to: OMP_AN:27501

Genotype differences:

+lacZ4787(del)( +nlpI775(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Dibucaine
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:247956) Dibucaine

  • chemical concentration= 1.2 mM
  • normalized fitness= -5.46

OMP_AN:33019

OMP:0007879

decreased resistance to an antimicrobial agent

Relative to: OMP_AN:27550

Genotype differences:

+lacZ4787(del)( +nlpI775(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Ethidium Bromide
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:4883) Ethidium Bromide

  • chemical concentration= 10 ug/ml
  • normalized fitness= -3.72

OMP_AN:33020

OMP:0006044

decreased resistance to beta-lactam

Relative to: OMP_AN:27533

Genotype differences:

+lacZ4787(del)( +nlpI775(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Carbenicillin disodium
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34609) Carbenicillin disodium

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.43

OMP_AN:33021

OMP:0006061

decreased resistance to tetracycline

Relative to: OMP_AN:27522

Genotype differences:

+lacZ4787(del)( +nlpI775(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Doxycycline hyclate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:34730) Doxycycline hyclate

  • chemical concentration= 1 ug/ml
  • normalized fitness= -4.31

OMP_AN:33022

OMP:0006057

decreased resistance to aminoglycoside

Relative to: OMP_AN:27527

Genotype differences:

+lacZ4787(del)( +nlpI775(del) -lacZ4787(del)

Condition differences:

no differences

  • temperature:37C
  • medium:LB agar
  • medium:Spectinomycin dihydrochloride pentahydrate
 ECO:0007005

high throughput direct assay evidence used in manual assertion

PMID:21185072[2]

towards some (CHEBI:9217) Spectinomycin dihydrochloride pentahydrate

  • chemical concentration= 4 ug/ml
  • normalized fitness= -3.68
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Additional Information

Links

References

See Help:References for how to manage references in OMPwiki.

  1. ↑ Inoue, T et al. (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189 950-7 PubMed OMPwiki page
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 Nichols, RJ et al. (2011) Phenotypic landscape of a bacterial cell. Cell 144 143-56 PubMed OMPwiki page
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