PMID:16591520
| Citation |
Fox, CF, Carter, JR and Kennedy, EP (1967) GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli.Proc. Natl. Acad. Sci. U.S.A. 57:698-705 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
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Main Points of the Paper
- The M protein of β-galactoside transport is the product of lacY
- M is localized in the spheroplast membrane fraction of the cell
- Estimate: xx functional subunits of M protein /cell
Materials and Methods Used
- Strain: ML30 (i+z+y+a+)
- Strain: ML308-225 (i-z-y+a+)- a derivative of ML30
- Strain: 3000 (i+z+y+a+)
- Strain: YA470 (i+z+y-)- y- amber mutant
- Strain: CA7047 (i+z+y-a+)- y- amber mutant
- Strain: CA8202 (i+z+y-a+)- y- amber mutant
- Strain: CA8204 (i+z+y-a+)- y- amber mutant
- Strain: MS1054 (i+z+y+a+)- y- deletion
- Strain: MS356 (i+z+y-a+)- y- deletion
- Strain: MS1019 (i+z+y-a+)- y- deletion
- Strain: MS1068 (i+z+y-a+)- y- deletion
- Strain: A 279A (i+z+ya+)
- Preparation of membrane-containing particulate fraction
- Unlabeled N-ethylmaleimide treatment
- M protein assay
Phenotype Annotations
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
ML308 |
lacI- |
constitutive expression of M protein (LacY) |
Other |
membrane particulate fractions contain the M protein regardless of induction status |
Other |
Table 1- protein-bound NEM |
|
Escherichia coli |
NCBI:562 |
ML3 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 1- NEM binding |
|
Escherichia coli |
NCBI:562 |
ML35 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 1- NEM binding |
|
Escherichia coli K12 |
NCBI:83333 |
YA470 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 2- NEM binding |
|
Escherichia coli K12 |
NCBI:83333 |
CA7047 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 2- NEM binding |
|
Escherichia coli K12 |
NCBI:83333 |
CA8202 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 2- NEM binding |
|
Escherichia coli K12 |
NCBI:83333 |
CA8204-ts 3 |
lacY |
temperature sensitive growth on lactose |
Growth |
exhibited lac+ phenotype at 25C, but appeared lac- when grown at 42C |
Plating Assay |
Figure 1- ONPG hydrolysis in vivo |
|
Escherichia coli K12 |
NCBI:83333 |
MS1054 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 2- NEM binding |
|
Escherichia coli K12 |
NCBI:83333 |
MS1068 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 2- NEM binding |
|
Escherichia coli K12 |
NCBI:83333 |
MS356 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 2- NEM binding |
|
Escherichia coli K12 |
NCBI:83333 |
MS1019 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 2- NEM binding |
|
Escherichia coli K12 |
NCBI:83333 |
CA8204 |
lacY- |
absence of substrate binding |
Other |
no detectable M protein in membrane particulate fractions |
Other |
Table 2- NEM binding |
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