PMID:16591520

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Citation

Fox, CF, Carter, JR and Kennedy, EP (1967) GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli.Proc. Natl. Acad. Sci. U.S.A. 57:698-705

Abstract

No abstract in PubMed

Links

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Keywords


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Main Points of the Paper

  • The M protein of β-galactoside transport is the product of lacY
  • M is localized in the spheroplast membrane fraction of the cell
  • Estimate: xx functional subunits of M protein /cell

Materials and Methods Used

  • Strain: ML30 (i+z+y+a+)
  • Strain: ML308-225 (i-z-y+a+)- a derivative of ML30
  • Strain: 3000 (i+z+y+a+)
  • Strain: YA470 (i+z+y-)- y- amber mutant
  • Strain: CA7047 (i+z+y-a+)- y- amber mutant
  • Strain: CA8202 (i+z+y-a+)- y- amber mutant
  • Strain: CA8204 (i+z+y-a+)- y- amber mutant
  • Strain: MS1054 (i+z+y+a+)- y- deletion
  • Strain: MS356 (i+z+y-a+)- y- deletion
  • Strain: MS1019 (i+z+y-a+)- y- deletion
  • Strain: MS1068 (i+z+y-a+)- y- deletion
  • Strain: A 279A (i+z+ya+)
  • Preparation of membrane-containing particulate fraction
  • Unlabeled N-ethylmaleimide treatment
  • M protein assay

Phenotype Annotations

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes
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Notes

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