PMID:5327656: Difference between revisions

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== Materials and Methods Used ==
== Materials and Methods Used ==
{{LitMaterials}}
*Parent Strain: ''Δlac su<sup>-</sup>''/ F''i<sup>S</sup>o<sup>+</sup>z<sup>+</sup>y<sup>+</sup>
**''i<sup>S</sup>''- dominant over ''i<sup>+</sup> and not inducible by lactose sz 43
*5 strains with suppressor mutations
**su<sup>-</sup>
**su<sup>+1</sup>
**su<sup>+2</sup>
**su<sup>+3</sup>
**su<sup>+4</sup>


==Phenotype Annotations==
==Phenotype Annotations==

Revision as of 22:43, 16 December 2010

Citation

Bourgeois, S, Cohn, M and Orgel, LE (1965) Suppression of and complementation among mutants of the regulatory gene of the lactose operon of Escherichia coli.J. Mol. Biol. 14:300-2

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Escherichia coli; Genes; Genetic Code; Lactose; Molecular Biology; Mutation

Main Points of the Paper

  • The regulatory gene, i, codes for a protein whereas the operator gene, o, probably does not

Materials and Methods Used

  • Parent Strain: Δlac su-/ FiSo+z+y+
    • iS- dominant over i+ and not inducible by lactose sz 43
  • 5 strains with suppressor mutations
    • su-
    • su+1
    • su+2
    • su+3
    • su+4

Phenotype Annotations

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:562

Δlac su- /Fiso+z+y+

deficient in lactose metabolism (lac-)

Metabolic Activity

Biochemical Assay

LacZ assay


Notes