PMID:4895844

Citation	Hane, MW and Wood, TH (1969) Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies. J. Bacteriol. 99:238-41
Abstract	Escherichia coli K-12 strains tested so far (approximately 20) can be separated into three groups on the basis of their abilities to form colonies on nutrient agar supplemented with nalidixic acid (NAL): (i) Nal(s) or wild type (no growth at 1 to 2 mug/ml); (ii) NalA(r) (growth at 40 mug/ml or higher); and (iii) NalB(r) (growth at 4 mug/ml, but no growth at 10 mug/ml). The NalA(r) group has a spectrum of sensitivity ranging from 60 to over 100 mug/ml. All Hfr strains of the NalA(r) and NalB(r) groups transfer NAL resistance to recipient cells at genetic loci which are at 42.5 +/- 0.5 and 51 +/- 1 min, respectively, on the Taylor-Trotter map. Some members of the NalA(r) group also have the genetic locus for NalB(r). The nalA(s) allele is completely dominant to nalA(r) in a partial diploid configuration. In haploids, nalA(r)-nalB(r) is phenotypically NalA(r); nalA(r)-nalB(s) is NalA(r); and nalA(s)-nalB(r) is NalB(r). The map location of nalA and the easy differentiation between NalA(r) and NalA(s) allow this marker to be used as a counterselector in bacterial conjugation experiments.
Links	PubMed PMC249993
Keywords	Alleles; Chromosome Mapping; Conjugation, Genetic; Drug Resistance, Microbial; Escherichia coli/drug effects; Genes, Dominant; Genetics, Microbial; Mutation; Nalidixic Acid/pharmacology
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: escherichia coli Strain: k-12 Substrain: KL16	Genotype of Reference Strain: nalA Genotype of Experimental Strain : nalA mutation in strain MH5	Reference Condition:					resistance to the nalidixic acid, experiment done as antibacterial assay. found similar resistance to concentration in strain MH7. sensitive at 60ug
a mutation or genetic difference within a strain	Taxon: escherichia coli Strain: k-12 Substrain: KL16	Genotype of Reference Strain: nalA Genotype of Experimental Strain : nalA mutation in strain MH6	Reference Condition:					resistance to the nalidixic acid, experiment done as antibacterial assay. sensitive at 100ug
a mutation or genetic difference within a strain	Taxon: escherichia coli Strain: k-12	Genotype of Reference Strain: nalA Genotype of Experimental Strain : nalA mutation in strain MH1004	Reference Condition:					resistance to the nalidixic acid, experiment done as antibacterial assay. MH1004 is a partial diploid carrying nalAs/nalAr and that sensitivity to NAL is completely dominant to resistance
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