PMID:16591520: Difference between revisions
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*Strain: MS1068 (''i<sup>+</sup>z<sup>+</sup>y<sup>-</sup>a<sup>+</sup>'')- y<sup>-</sup> deletion | *Strain: MS1068 (''i<sup>+</sup>z<sup>+</sup>y<sup>-</sup>a<sup>+</sup>'')- y<sup>-</sup> deletion | ||
*Strain: A 279A (''i<sup>+</sup>z<sup>+</sup>y<sup></sup>a<sup>+</sup>'') | *Strain: A 279A (''i<sup>+</sup>z<sup>+</sup>y<sup></sup>a<sup>+</sup>'') | ||
*Preparation of membrane-containing particulate fraction | |||
*Unlabeled N-ethylmaleimide treatment | |||
*M protein assay | |||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
Revision as of 22:33, 13 December 2010
| Citation |
Fox, CF, Carter, JR and Kennedy, EP (1967) GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli.Proc. Natl. Acad. Sci. U.S.A. 57:698-705 |
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| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
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| edit table |
Main Points of the Paper
- The M protein of β-galactoside transport is the product of lacY
- M is localized in the spheroplast membrane fraction of the cell
- Estimate: xx functional subunits of M protein /cell
Materials and Methods Used
- Strain: ML30 (i+z+y+a+)
- Strain: ML308-225 (i-z-y+a+)- a derivative of ML30
- Strain: 3000 (i+z+y+a+)
- Strain: YA470 (i+z+y-)- y- amber mutant
- Strain: CA7047 (i+z+y-a+)- y- amber mutant
- Strain: CA8202 (i+z+y-a+)- y- amber mutant
- Strain: CA8204 (i+z+y-a+)- y- amber mutant
- Strain: MS1054 (i+z+y+a+)- y- deletion
- Strain: MS356 (i+z+y-a+)- y- deletion
- Strain: MS1019 (i+z+y-a+)- y- deletion
- Strain: MS1068 (i+z+y-a+)- y- deletion
- Strain: A 279A (i+z+ya+)
- Preparation of membrane-containing particulate fraction
- Unlabeled N-ethylmaleimide treatment
- M protein assay
Phenotype Annotations
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
| edit table |
