PMID:230407

Citation	Daniel, J and Danchin, A (1979) Involvement of cyclic AMP and its receptor protein in the sensitivity of Escherichia coli K 12 toward serine: excretion of 2-ketobutyrate, a precursor of isoleucine.Mol. Gen. Genet. 176:343-50
Abstract	A relationship between serine-induced growth sensitivity and the cAMP-CAP complex is established. Mutants of Escherichia coli K 12 deficient either in the cya or crp gene function exhibit a resistant phenotype on serine media although they harbor a relA allele normally leading to sensitivity toward serine. The presence of a crp allele in a cya delta relA background restores the sensitivity phenotype, while the analysis of serine resistant mutants selected from a crp cya delta relA strain shows that the mutation leading to resistance is located at, or very near, the crp gene, giving a more or less Crp- phenotype. In addition crp cya delta relA strains excrete large quantities of 2-ketobutyrate when grown on glucose M63 medium. This excretion is unambiguously linked to the presence of the crp allele and is correlated with an enhanced threonine deaminase activity. Besides, the complex regulation exerted on the acetolactate synthase activities is discussed.
Links	PubMed
Keywords	Butyrates; Cyclic AMP; Escherichia coli; Isoleucine; Mutation; Phenotype; Receptors, Cyclic AMP; Serine; Transduction, Genetic
edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CA8307 NCBI Taxon ID: 83333	Genotype of Reference Strain: crp relA Genotype of Experimental Strain : crp - relA +	Reference Condition:					Deficient mutants in relA backgroumd, were found to be resistant to smg (serine+methionine+glycine)
edit table

References

See Help:References for how to manage references in OMPwiki.