Difference between revisions of "PMID:18621901"

From OMPwiki
(New PMID: Page!)
 
(Fill PMID: Page!)
 
Line 1: Line 1:
 +
{{RightTOC}}
  
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2897.F4f745e35e5a35-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="F4f745e35e5a35"  class=" tableEdit PMID_info_table" 
 +
 +
|-
 +
!align=left  |Citation
 +
||
 +
'''Tamae, C, Liu, A, Kim, K, Sitz, D, Hong, J, Becket, E, Bui, A, Solaimani, P, Tran, KP, Yang, H and Miller, JH'''  (2008) Determination of antibiotic hypersensitivity among 4,000 single-gene-knockout mutants of Escherichia coli.''J. Bacteriol.'' '''190''':5981-8
 +
|-
 +
!align=left  |Abstract
 +
||
 +
We have tested the entire Keio collection of close to 4,000 single-gene knockouts in Escherichia coli for increased susceptibility to one of seven different antibiotics (ciprofloxacin, rifampin, vancomycin, ampicillin, sulfamethoxazole, gentamicin, or metronidazole). We used high-throughput screening of several subinhibitory concentrations of each antibiotic and reduced more than 65,000 data points to a set of 140 strains that display significantly increased sensitivities to at least one of the antibiotics, determining the MIC in each case. These data provide targets for the design of "codrugs" that can potentiate existing antibiotics. We have made a number of double mutants with greatly increased sensitivity to ciprofloxacin, and these overcome the resistance generated by certain gyrA mutations. Many of the gene knockouts in E. coli are hypersensitive to more than one antibiotic. Together, all of these data allow us to outline the cell's "intrinsic resistome," which provides innate resistance to antibiotics.
 +
|-
 +
!align=left  |Links
 +
||
 +
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18621901 PubMed]
 +
Online version:[http://dx.doi.org/10.1128/JB.01982-07 10.1128/JB.01982-07]
 +
|-
 +
!align=left  |Keywords
 +
||
 +
Ampicillin; Anti-Bacterial Agents; Ciprofloxacin; Escherichia coli; Genes, Bacterial; Gentamicins; Metronidazole; Microbial Sensitivity Tests; Mutation; Rifampin; Sulfamethoxazole; Vancomycin
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2897.F4f745e35e5a35&page=2897&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2897.F4f745e35e5a35-->
 +
 +
==Main Points of the Paper ==
 +
{{LitSignificance}}
 +
 +
== Materials and Methods Used ==
 +
{{LitMaterials}}
 +
 +
==Phenotype Annotations==
 +
{{AnnotationTableHelp}}
 +
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.2897.W4f745e36014ad-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="W4f745e36014ad"  class=" tableEdit Phenotype_Table_2" 
 +
|-
 +
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2897.W4f745e36014ad&page=2897&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2897.W4f745e36014ad--></protect>
 +
 +
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 +
<references/>
 +
 +
 +
[[Category:Publication]]

Latest revision as of 07:05, 29 March 2012

Citation

Tamae, C, Liu, A, Kim, K, Sitz, D, Hong, J, Becket, E, Bui, A, Solaimani, P, Tran, KP, Yang, H and Miller, JH (2008) Determination of antibiotic hypersensitivity among 4,000 single-gene-knockout mutants of Escherichia coli.J. Bacteriol. 190:5981-8

Abstract

We have tested the entire Keio collection of close to 4,000 single-gene knockouts in Escherichia coli for increased susceptibility to one of seven different antibiotics (ciprofloxacin, rifampin, vancomycin, ampicillin, sulfamethoxazole, gentamicin, or metronidazole). We used high-throughput screening of several subinhibitory concentrations of each antibiotic and reduced more than 65,000 data points to a set of 140 strains that display significantly increased sensitivities to at least one of the antibiotics, determining the MIC in each case. These data provide targets for the design of "codrugs" that can potentiate existing antibiotics. We have made a number of double mutants with greatly increased sensitivity to ciprofloxacin, and these overcome the resistance generated by certain gyrA mutations. Many of the gene knockouts in E. coli are hypersensitive to more than one antibiotic. Together, all of these data allow us to outline the cell's "intrinsic resistome," which provides innate resistance to antibiotics.

Links

PubMed Online version:10.1128/JB.01982-07

Keywords

Ampicillin; Anti-Bacterial Agents; Ciprofloxacin; Escherichia coli; Genes, Bacterial; Gentamicins; Metronidazole; Microbial Sensitivity Tests; Mutation; Rifampin; Sulfamethoxazole; Vancomycin

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
edit table

Notes

References

See Help:References for how to manage references in OMPwiki.

Retrieved from "https://microbialphenotypes.org/wiki/index.php?title=PMID:18621901&oldid=6686"