PMID:14168696: Difference between revisions
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== Materials and Methods Used == | == Materials and Methods Used == | ||
*Mutant Classes | |||
**''o<sup>c</sup>'' mutants | |||
***lost the sensitivity to repressor, therefore expressing β-galactosidase constitutively in the presence of the repressor | |||
*** very closely linked to ''z''- mutations mapped at the beginning of the ''z'' gene | |||
**''o<sup>o</sup>'' mutants | |||
***shuts off the production of all 3 enzymic activities of the ''lac'' operon | |||
*Selection of ''z-y+'' revertants on melibiose-agar | |||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
Revision as of 20:17, 22 November 2010
| Citation |
BECKWITH, JR (1964) A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.J. Mol. Biol. 8:427-30 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Deletion; Lac; Operator; Escherichia coli; Galactosidases; Genetics; Membrane Transport Proteins; Mutation; RNA, Bacterial; Research; Enzymes; Revertants; Constitutive |
| edit table |
Main Points of the Paper
- The lac operator does not lie in the same region in which oo mutants are found.
Materials and Methods Used
- Mutant Classes
- oc mutants
- lost the sensitivity to repressor, therefore expressing β-galactosidase constitutively in the presence of the repressor
- very closely linked to z- mutations mapped at the beginning of the z gene
- oo mutants
- shuts off the production of all 3 enzymic activities of the lac operon
- oc mutants
- Selection of z-y+ revertants on melibiose-agar
Phenotype Annotations
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
| edit table |
