Difference between revisions of "PMID:20727918"

From OMPwiki
(Table edited by Tkveton via TableEdit)
(Table edited by Tkveton via TableEdit)
Line 345: Line 345:
 
NfsA (Ec)= 2-NO<sub>2</sub> nitroreductase only, from E. coli;
 
NfsA (Ec)= 2-NO<sub>2</sub> nitroreductase only, from E. coli;
 
phenotype is unchanged
 
phenotype is unchanged
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2;
 +
NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli
 
|
 
|
 
   
 
   
Line 372: Line 398:
 
NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae;
 
NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae;
 
phenotype is unchanged
 
phenotype is unchanged
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2;
 +
NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia
 
|
 
|
 
   
 
   
Line 399: Line 451:
 
NfsB= 2-NO2 or 4-NO2 nitroreductase;
 
NfsB= 2-NO2 or 4-NO2 nitroreductase;
 
phenotype is unchanged
 
phenotype is unchanged
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsB uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2;
 +
NfsB= 2-NO2 or 4-NO2 nitroreductase
 
|
 
|
 
   
 
   
Line 426: Line 504:
 
NemA= 4-NO2 nitroreductase only;
 
NemA= 4-NO2 nitroreductase only;
 
phenotype is unchanged
 
phenotype is unchanged
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: SOS-R1
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NemA uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2;
 +
NemA= 4-NO2 nitroreductase only
 
|
 
|
 
   
 
   
Line 464: Line 568:
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
+
*Genotype of Reference Strain: ''SOS-R1 YfkO uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
 
|
 
|
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
Line 478: Line 582:
 
|
 
|
 
Reference figure 2;  
 
Reference figure 2;  
−
NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli
+
Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsB uvrB(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NemA uvrB(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; NemA= 4-NO2 nitroreductase only
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 YfkO uvrB(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsA mutS(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsA mutS(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsB mutS(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NemA mutS(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; NemA= 4-NO2 nitroreductase only
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 YfkO mutS(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsA ada(del) ogt(del)''
 +
|
 +
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
 +
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli
 
|
 
|
 
   
 
   
Line 486: Line 909:
 
|
 
|
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 +
 
*Strain: K-12
 
*Strain: K-12
 +
 
*Substrain: SOS-R1
 
*Substrain: SOS-R1
−
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
+
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
+
*Genotype of Reference Strain: ''SOS-R1 NfsA ada(del) ogt(del)''
 
|
 
|
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 
|
 
|
Line 503: Line 930:
 
LacZ transcript localization evidence
 
LacZ transcript localization evidence
 
|
 
|
−
Reference figure 2;  
+
Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia
−
NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia
 
 
|
 
|
 
   
 
   
Line 512: Line 938:
 
|
 
|
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 +
 
*Strain: K-12
 
*Strain: K-12
 +
 
*Substrain: SOS-R1
 
*Substrain: SOS-R1
−
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
+
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''SOS-R1 NfsB uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
+
*Genotype of Reference Strain: ''SOS-R1 NfsB ada(del) ogt(del)''
 
|
 
|
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 
|
 
|
Line 529: Line 959:
 
LacZ transcript localization evidence
 
LacZ transcript localization evidence
 
|
 
|
−
Reference figure 2;  
+
Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase
−
NfsB= 2-NO2 or 4-NO2 nitroreductase
 
 
|
 
|
 
   
 
   
Line 538: Line 967:
 
|
 
|
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 +
 
*Strain: K-12
 
*Strain: K-12
 +
 
*Substrain: SOS-R1
 
*Substrain: SOS-R1
−
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
+
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''SOS-R1 NemA uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
+
*Genotype of Reference Strain: ''SOS-R1 NemA ada(del) ogt(del)''
 
|
 
|
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 
|
 
|
Line 555: Line 988:
 
LacZ transcript localization evidence
 
LacZ transcript localization evidence
 
|
 
|
−
Reference figure 2;  
+
Reference figure 2; NemA= 4-NO2 nitroreductase only
−
NemA= 4-NO2 nitroreductase only
 
 
|
 
|
 
   
 
   
Line 564: Line 996:
 
|
 
|
 
*Taxon: Escherichia coli
 
*Taxon: Escherichia coli
 +
 
*Strain: K-12
 
*Strain: K-12
 +
 
*Substrain: SOS-R1
 
*Substrain: SOS-R1
−
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
+
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 
|
 
|
−
*Genotype of Reference Strain: ''SOS-R1 YfkO uvrB<sup>+</sup> mutS<sup>+</sup> ada<sup>+</sup> ogt<sup>+</sup>''
+
*Genotype of Reference Strain: ''SOS-R1 YfkO ada(del) ogt(del)''
 
|
 
|
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 
*Reference Condition: -CB1954 (0 micromolar for 3 hours)
 +
 
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 
*Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 
|
 
|
Line 581: Line 1,017:
 
LacZ transcript localization evidence
 
LacZ transcript localization evidence
 
|
 
|
−
Reference figure 2;  
+
Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis
−
Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis
+
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup>''
 +
 
 +
*Genotype of Experimental Strain: ‘’SOS-R1 NfsA uvrB(del)’’
 +
|
 +
*Reference Condition:  5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 3; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsA uvrB<sup>+</sup>''
 +
 
 +
*Genotype of Experimental Strain: ‘’SOS-R1 NfsA uvrB(del)’’
 +
|
 +
*Reference Condition:  5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 3; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NfsB uvrB<sup>+</sup>''
 +
 
 +
*Genotype of Experimental Strain: ‘’SOS-R1 NfsB uvrB(del)’’
 +
|
 +
*Reference Condition:  5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 3; NfsB= 2-NO2 or 4-NO2 nitroreductase
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 NemA uvrB<sup>+</sup>''
 +
 
 +
*Genotype of Experimental Strain: ‘’SOS-R1 NemA uvrB(del)’’
 +
|
 +
*Reference Condition:  5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 3; NemA= 4-NO2 nitroreductase only
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
 
 +
*Strain: K-12
 +
 
 +
*Substrain: SOS-R1
 +
 
 +
*[http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
 +
|
 +
*Genotype of Reference Strain: ''SOS-R1 YfkO uvrB<sup>+</sup>''
 +
 
 +
*Genotype of Experimental Strain: ‘’SOS-R1 YfkO uvrB(del)’’
 +
|
 +
*Reference Condition:  5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 +
|
 +
OMP:0005024
 +
|
 +
increased induced expression of the SOS response
 +
|
 +
ECO:0000297
 +
|
 +
LacZ transcript localization evidence
 +
|
 +
Reference figure 3; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis
 
|
 
|
 
   
 
   

Revision as of 12:38, 26 February 2015

Citation

Prosser, GA, Patterson, AV and Ackerley, DF (2010) uvrB gene deletion enhances SOS chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug CB1954.J. Biotechnol. 150:190-4

Abstract

CB1954 is an anti-cancer prodrug that can be reduced at either of two nitro groups to form cytotoxic metabolites. We describe here two efficient and previously uncharacterized nitroreductases, YfkO from Bacillus subtilis which reduces CB1954 exclusively at the 4-NO(2) position, and NfsA from Klebsiella pneumoniae which preferentially reduces the 2-NO(2) group. Utilizing these novel enzymes, together with three previously characterized nitroreductases, we demonstrate that the Escherichia coli SOS-chromotest assay can differentially detect the 4-nitro versus 2-nitro reduction products of CB1954 following deletion of the nucleotide excision repair gene uvrB, but not mismatch repair (mutS) or methyltransferase (ada/ogt) genes. These findings may hold significance for identification and selection of nitroreductases for CB1954-mediated gene therapy, particularly when targeting tumors that are deficient in nucleotide excision repair. Moreover, we demonstrate that comparative SOS chromotest analysis in wild type and uvrB mutant strains can be used to determine whether or not nucleotide excision repair plays a significant role in processing DNA damage resulting from activation of different nitroaromatic prodrugs.

Links

PubMed Online version:10.1016/j.jbiotec.2010.08.007

Keywords

Antineoplastic Agents; Aziridines; Bacillus subtilis; Bacterial Proteins; DNA Helicases; Escherichia coli Proteins; Gene Deletion; Kinetics; Klebsiella pneumoniae; Metronidazole; Molecular Conformation; Molecular Sequence Data; Nitrofurazone; Nitroreductases; SOS Response (Genetics)

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA uvrB+
  • Genotype of Experimental Strain : SOS-R1 pNfsA uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA uvrB+
  • Genotype of Experimental Strain : SOS-R1 pNfsA uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsB uvrB+
  • Genotype of Experimental Strain : SOS-R1 pNfsB uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNemA uvrB+
  • Genotype of Experimental Strain : SOS-R1 pNemA uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NemA= 4-NO2 nitroreductase only

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pYfkO uvrB+
  • Genotype of Experimental Strain : SOS-R1 pYfkO uvrB(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA mutS+
  • Genotype of Experimental Strain : SOS-R1 pNfsA mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA mutS+
  • Genotype of Experimental Strain : SOS-R1 pNfsA mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsB mutS+
  • Genotype of Experimental Strain : SOS-R1 pNfsB mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNemA mutS+
  • Genotype of Experimental Strain : SOS-R1 pNemA mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NemA= 4-NO2 nitroreductase only; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pYfkO mutS+
  • Genotype of Experimental Strain : SOS-R1 pYfkO mutS(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pNfsA ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 NfsA uvrB+ mutS+ ada+ ogt+
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsA ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pNfsA ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumoniae; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 NfsA uvrB+ mutS+ ada+ ogt+
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNfsB ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pNfsB ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 NfsB uvrB+ mutS+ ada+ ogt+
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pNemA ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pNemA ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NemA= 4-NO2 nitroreductase only; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 NemA uvrB+ mutS+ ada+ ogt+
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NemA= 4-NO2 nitroreductase only

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 pYfkO ada+ ogt+
  • Genotype of Experimental Strain : SOS-R1 pYfkO ada(del) ogt(del)
  • Reference Condition: +CB1954 (50 micromolar for 3 hours) compared to 0 micromolar CB1954
 OMP:0005023

presence of an SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis; phenotype is unchanged

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: SOS-R1 YfkO uvrB+ mutS+ ada+ ogt+
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsA uvrB(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsA uvrB(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsB uvrB(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NemA uvrB(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NemA= 4-NO2 nitroreductase only

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 YfkO uvrB(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsA mutS(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsA mutS(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsB mutS(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NemA mutS(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NemA= 4-NO2 nitroreductase only

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 YfkO mutS(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsA ada(del) ogt(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsA ada(del) ogt(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsB ada(del) ogt(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NfsB= 2-NO2 or 4-NO2 nitroreductase

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NemA ada(del) ogt(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; NemA= 4-NO2 nitroreductase only

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 YfkO ada(del) ogt(del)
  • Reference Condition: -CB1954 (0 micromolar for 3 hours)
  • Experimental Condition: +CB1954 (50 micromolar for 3 hours)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 2; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsA uvrB+
  • Genotype of Experimental Strain: ‘’SOS-R1 NfsA uvrB(del)’’
  • Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 3; NfsA (Ec)= 2-NO2 nitroreductase only, from E. coli

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsA uvrB+
  • Genotype of Experimental Strain: ‘’SOS-R1 NfsA uvrB(del)’’
  • Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 3; NfsA (Kp)= 2-NO2 nitroreductase only, from K. pneumonia

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NfsB uvrB+
  • Genotype of Experimental Strain: ‘’SOS-R1 NfsB uvrB(del)’’
  • Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 3; NfsB= 2-NO2 or 4-NO2 nitroreductase

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 NemA uvrB+
  • Genotype of Experimental Strain: ‘’SOS-R1 NemA uvrB(del)’’
  • Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 3; NemA= 4-NO2 nitroreductase only

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SOS-R1
  • Genotype of Reference Strain: SOS-R1 YfkO uvrB+
  • Genotype of Experimental Strain: ‘’SOS-R1 YfkO uvrB(del)’’
  • Reference Condition: 5-nitrofuran nitrofurazone (5 micromolar for 3 hours compared to 0 micromolar)
 OMP:0005024

increased induced expression of the SOS response

ECO:0000297

LacZ transcript localization evidence

Reference figure 3; Yfko (Bs)= 4-NO2 nitroreductase only, from B. licheniformis


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Notes

References

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