Difference between revisions of "PMID:20004108"
(Table edited by Joncrz via TableEdit) |
(Table edited by Tkveton via TableEdit) |
||
| Line 48: | Line 48: | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** --> | ||
| − | {| | + | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U4f1877d51c72d" class=" tableEdit Phenotype_Table_2" |
| − | |- | + | |- align='left' bgcolor='#CCCCFF' |
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
|- | |- | ||
| Line 60: | Line 60: | ||
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333] | *NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333] | ||
| | | | ||
| − | *Genotype of Reference Strain: ''uvrB'' | + | *Genotype of Reference Strain: ''uvrB<sup>+</sup>'' |
*Genotype of Experimental Strain : ''uvrB5'' | *Genotype of Experimental Strain : ''uvrB5'' | ||
| | | | ||
*Reference Condition: | *Reference Condition: | ||
| | | | ||
| − | + | ||
| | | | ||
| − | + | ||
| | | | ||
| Line 73: | Line 73: | ||
| | | | ||
| − | + | uvrB5 causes hypersensitivity to PUVA (UVA-A light) treatments performed with both mono-functional 3-carbethoxypsoralen and bifunctional 8-Monofunctional psoralens when compared to wildtype, figure 1 & 2. | |
| | | | ||
| Line 80: | Line 80: | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2828.U4f1877d51c72d&page=2828&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || || | |<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.2828.U4f1877d51c72d&page=2828&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || || | ||
|} | |} | ||
| + | <noinclude> | ||
| + | |||
| + | </noinclude> | ||
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.2828.U4f1877d51c72d--></protect> | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.2828.U4f1877d51c72d--></protect> | ||
Revision as of 11:50, 22 January 2015
| Citation |
Lage, C, Gonçalves, SR, Souza, LL, de Pádula, M and Leitão, AC (2010) Differential survival of Escherichia coli uvrA, uvrB, and uvrC mutants to psoralen plus UV-A (PUVA): Evidence for uncoupled action of nucleotide excision repair to process DNA adducts.J. Photochem. Photobiol. B, Biol. 98:40-7 |
|---|---|
| Abstract |
The nucleotide excision repair mechanism (NER) of Escherichia coli is responsible for the recognition and elimination of more than twenty different DNA lesions. Herein, we evaluated the in vivo role of NER in the repair of DNA adducts generated by psoralens (mono- or bi-functional) and UV-A light (PUVA) in E. coli. Cultures of wild-type E. coli K12 and mutants for uvrA, uvrB, uvrC or uvrAC genes were treated with PUVA and cell survival was determined. In parallel, kinetics of DNA repair was also evaluated by the comparison of DNA sedimentation profiles in all the strains after PUVA treatment. The uvrB mutant was more sensitive to PUVA treatment than all the other uvr mutant strains. Wild-type strain, and uvrA and uvrC mutants were able to repair PUVA-induced lesions, as seen by DNA sedimentation profiles, while the uvrB mutant was unable to repair the lesions. In addition, a quadruple fpg nth xth nfo mutant was unable to nick PUVA-treated DNA when the crude cell-free extract was used to perform plasmid nicking. These data suggest that DNA repair of PUVA-induced lesions may require base excision repair functions, despite proficient UvrABC activity. These results point to a specific role for UvrB protein in the repair of psoralen adducts, which appear to be independent of UvrA or UvrC proteins, as described for the classical UvrABC endonuclease mechanism. |
| Links |
PubMed Online version:10.1016/j.jphotobiol.2009.11.001 |
| Keywords |
Adenosine Triphosphatases; DNA Adducts; DNA Helicases; DNA Repair; DNA-Binding Proteins; Endodeoxyribonucleases; Escherichia coli; Escherichia coli Proteins; Ficusin; Kinetics; Mutant Proteins; Photosensitizing Agents; Ultraviolet Rays |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
uvrB5 causes hypersensitivity to PUVA (UVA-A light) treatments performed with both mono-functional 3-carbethoxypsoralen and bifunctional 8-Monofunctional psoralens when compared to wildtype, figure 1 & 2. |
| ||||
| edit table |
Notes
References
See Help:References for how to manage references in OMPwiki.
