Difference between revisions of "PMID:18820021"

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'''Krishnan, K and Flower, AM'''  (2008) Suppression of DeltabipA phenotypes in Escherichia coli by abolishment of pseudouridylation at specific sites on the 23S rRNA. ''J. Bacteriol.'' '''190''':7675-83
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!align=left align='left' bgcolor='#CCCCFF' |Abstract
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The BipA protein of Escherichia coli has intriguing similarities to the elongation factor subfamily of GTPases, including EF-Tu, EF-G, and LepA. In addition, phenotypes of a bipA deletion mutant suggest that BipA is involved in regulation of a variety of pathways. These two points have led to speculation that BipA may be a novel regulatory protein that affects efficient translation of target genes through direct interaction with the ribosome. We isolated and characterized suppressors of the cold-sensitive growth phenotype exhibited by DeltabipA strains and identified insertion mutations in rluC. The rluC gene encodes a pseudouridine synthase responsible for pseudouridine modification of 23S rRNA at three sites, all located near the peptidyl transferase center. Deletion of rluC not only suppressed cold sensitivity but also alleviated the decrease in capsule synthesis exhibited by bipA mutants, suggesting that the phenotypic effects of BipA are manifested through an effect on the ribosome. The suppressor effect is specific to rluC, as deletion of other rlu genes did not relieve cold sensitivity, and further, more than a single pseudouridine residue is involved, as alteration of single residues did not produce suppressors. These results are consistent with a role for BipA in either the structure or the function of the ribosome and imply that wild-type ribosomes are dependent on BipA for efficient expression of target mRNAs and that the lack of pseudouridylation at these three sites renders the ribosomes BipA independent.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18820021 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583627 PMC2583627]
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Online version:[http://dx.doi.org/10.1128/JB.00835-08 10.1128/JB.00835-08]
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Cold Temperature; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; GTP Phosphohydrolases/genetics; GTP Phosphohydrolases/metabolism; Gene Expression Regulation, Bacterial; Hydro-Lyases/genetics; Hydro-Lyases/metabolism; Mutation; Phosphoproteins/genetics; Phosphoproteins/metabolism; Pseudouridine/metabolism; RNA, Bacterial/genetics; RNA, Ribosomal, 23S/genetics; Time Factors
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="W5c9cf6d333fe0"  class=" tableEdit Phenotype_Table_2" 
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 11:31, 28 March 2019

Citation

Krishnan, K and Flower, AM (2008) Suppression of DeltabipA phenotypes in Escherichia coli by abolishment of pseudouridylation at specific sites on the 23S rRNA. J. Bacteriol. 190:7675-83

Abstract

The BipA protein of Escherichia coli has intriguing similarities to the elongation factor subfamily of GTPases, including EF-Tu, EF-G, and LepA. In addition, phenotypes of a bipA deletion mutant suggest that BipA is involved in regulation of a variety of pathways. These two points have led to speculation that BipA may be a novel regulatory protein that affects efficient translation of target genes through direct interaction with the ribosome. We isolated and characterized suppressors of the cold-sensitive growth phenotype exhibited by DeltabipA strains and identified insertion mutations in rluC. The rluC gene encodes a pseudouridine synthase responsible for pseudouridine modification of 23S rRNA at three sites, all located near the peptidyl transferase center. Deletion of rluC not only suppressed cold sensitivity but also alleviated the decrease in capsule synthesis exhibited by bipA mutants, suggesting that the phenotypic effects of BipA are manifested through an effect on the ribosome. The suppressor effect is specific to rluC, as deletion of other rlu genes did not relieve cold sensitivity, and further, more than a single pseudouridine residue is involved, as alteration of single residues did not produce suppressors. These results are consistent with a role for BipA in either the structure or the function of the ribosome and imply that wild-type ribosomes are dependent on BipA for efficient expression of target mRNAs and that the lack of pseudouridylation at these three sites renders the ribosomes BipA independent.

Links

PubMed PMC2583627 Online version:10.1128/JB.00835-08

Keywords

Cold Temperature; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; GTP Phosphohydrolases/genetics; GTP Phosphohydrolases/metabolism; Gene Expression Regulation, Bacterial; Hydro-Lyases/genetics; Hydro-Lyases/metabolism; Mutation; Phosphoproteins/genetics; Phosphoproteins/metabolism; Pseudouridine/metabolism; RNA, Bacterial/genetics; RNA, Ribosomal, 23S/genetics; Time Factors

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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