PMID:330501

Citation	Hobson, AC, Gho, D and Müller-Hill, B (1977) Isolation, genetic analysis, and characterization of Escherichia coli mutants with defects in the lacY gene. J. Bacteriol. 131:830-8
Abstract	Five hundred thirty-five lacY mutants were isolated from an Escherichia coli strain carrying the lactose operon on an F' factor, either without mutagenesis or after mutagenesis with 2-aminopurine or N-methyl-N'-nitro-N-nitrosoguanidine. Crosses against 48 independently isolated deletions ending in the lacY gene divided the gene into 36 deletion groups. Suppressibility studies with 7 nonsense suppressor strains classified 276 mutants as nonsense mutants and 78 as missense (or nonsuppressible) mutants. One hundred seventy-nine mutants were "leaky" and could not be so allocated, and two were found to have small internal deletions. Nonsense mutants could in many cases be subdivided even within deletion groups on the basis of their suppressibility pattern, giving a total of 70 groups of nonsense mutants. Studies of these mutants allow the following conclusions: lactose and melibiose most probably do not have separate binding sites on the permease; the lacY region most likely consists of one cistron, and so both active transport and facilitated diffusion are functions of one protein; and finally, there is probably no small defined region of the permease responsible for energy coupling of transport. Furthermore, the strains and the analysis form the basis for a future functional study of the permease by biochemical techniques.
Links	PubMed PMC235538
Keywords	2-Aminopurine; Biological Transport, Active; Chromosome Mapping; Diffusion; Disaccharides/metabolism; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Galactosidases/metabolism; Genes; Lactose/metabolism; Membrane Transport Proteins/metabolism; Methylnitronitrosoguanidine; Mutagens; Mutation; Suppression, Genetic
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: lacY Genotype of Experimental Strain : lacY in strain MAD59-lacY	Reference Condition: 42C Experimental Condition: 42C					No growth when Malibose was sole carbon source at 42C.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: lacY Genotype of Experimental Strain : lacY in strain MAD59-lacY	Reference Condition: 42C Experimental Condition: 42C					No growth when Lactose was sole carbon source at 42C.
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References

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