PMID:5330222

Citation	Müller-Hill, B (1966) Suppressible regulator constitutive mutants of the lactose system in Escherichia coli.J. Mol. Biol. 15:374-6
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Enzyme Repression; Escherichia coli; Galactosidases; Genetic Code; Guanidines; Lactose; Molecular Biology; Mutation; Purines; RNA, Messenger
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Main Points of the Paper

• Suppressors of nonsense codons exist for the regulatory i gene of the lactose operon, thereby proving that the functional i product is a protein.
  • Rationale: suppression of nonsense codons into amino acids occurs at the translational level

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

• Mutagenesis of E.coli strain 112-12 (gal^-, cys^-, his^-, su)
  • Mutagen-producing agents: 2-aminopurine or N-methyl-N'-nitro-N-nitrosguanidine

• ONPG solution to select for lac constitutive mutants

Phenotype Annotations

Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli	NCBI:562	112-12lacc (gal-cys-his-su-)	lacI	decreased beta-galactosidase activity	Metabolic Activity	decreased beta-galactosidase activity in the su+ revertants compared to the su- parent	Biochemical Assay	LacZ assay
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