PMID:5330222: Difference between revisions

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==Main Points of the Paper ==
==Main Points of the Paper ==
{{LitSignificance}}
*Suppression of nonsense codons exists in the regulatory ''i'' gene of the lactose operon, thereby proving that the functional i product is a protein.


== Materials and Methods Used ==
== Materials and Methods Used ==

Revision as of 22:44, 22 November 2010

Citation

Müller-Hill, B (1966) Suppressible regulator constitutive mutants of the lactose system in Escherichia coli.J. Mol. Biol. 15:374-6

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Enzyme Repression; Escherichia coli; Galactosidases; Genetic Code; Guanidines; Lactose; Molecular Biology; Mutation; Purines; RNA, Messenger

Main Points of the Paper

  • Suppression of nonsense codons exists in the regulatory i gene of the lactose operon, thereby proving that the functional i product is a protein.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

  • Mutagenesis of E.coli strain 112-12 (gal-, cys-, his-, su)
    • Mutagen-producing agents: 2-aminopurine or N-methyl-N'-nitro-N-nitrosguanidine
  • ONPG solution to select for lac constitutive mutants

Phenotype Annotations

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes


Notes