PMID:1100857

Citation	Aswad, D and Koshland, DE (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants.J. Mol. Biol. 97:225-35
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium; Species Specificity
edit table

Main Points of the Paper

Please summarize the main points of the paper.

• Isolation of non-chemotactic mutants
• Description of the preformed liquid gradient technique
  • utilizes a preformed gradient in a vertical column of liquid medium
  • separates wild-type, non-motile, and non-motile but chemotactic into 3 regions
• None of the 72 mutants isolated exhibited a frequency of spontaneous tumbling
  • 68 exhibited smooth co-ordinated motility with no evidence of tumbling
  • 4 exhibited uncoordinated motility
• Results suggest there may be as many as 6 genetically distinct classes of che mutants in Salmonella

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

• For the isolation of non-chemotactic mutants
  • Diethylsulfate mutagenesis
  • negative swarm selection on Tryptone semi-solid medium (0.3% agar)
  • preformed liquid gradient technique (described in detail)

• For complementations and strain constructions
  • Alternative method of abortive transduction

Phenotype Annotations

Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
edit table