Difference between revisions of "PMID:374384"
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| + | !align=left align='left' bgcolor='#CCCCFF' |Citation | ||
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| + | '''Itikawa, H and Ryu, J''' (1979) Isolation and characterization of a temperature-sensitive dnaK mutant of Escherichia coli B. ''J. Bacteriol.'' '''138''':339-44 | ||
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| + | !align=left align='left' bgcolor='#CCCCFF' |Abstract | ||
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| + | A temperature-sensitive dnaK mutant (strain MT112) was isolated from Escherichia coli B strain H/r30RT by thymineless death selection at 43 degrees C. By genetic mapping, the mutation [dnaK7(Ts)] was located near the thr gene (approximately 0.2 min on the may). E. coli K-12 transductants of the mutation to temperature sensitivity were assayed for their susceptibility to transducing phage lambda carrying the dnaK and/or the dnaJ gene. All of the transductants were able to propagate phage lambda carrying the dnaK gene. When macromolecular synthesis of the mutant was assayed at 43 degrees C, it was observed that both deoxyribonucleic acid and ribonucleic acid syntheses were severely inhibited. Thus, it was suggested that the conditionally defective dnaK mutation affects both cellular deoxyribonucleic acid and ribonucleic acid syntheses at the nonpermissive temperature in addition to inability to propagate phage lambda at permissive temperature. | ||
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| + | !align=left align='left' bgcolor='#CCCCFF' |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=374384 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC218183 PMC218183] | ||
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| + | !align=left align='left' bgcolor='#CCCCFF' |Keywords | ||
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| + | Chromosome Mapping; Coliphages/genetics; DNA, Bacterial/biosynthesis; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Lysogeny; Mutation; RNA, Bacterial/biosynthesis; Temperature; Transduction, Genetic | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Latest revision as of 14:18, 21 July 2015
| Citation |
Itikawa, H and Ryu, J (1979) Isolation and characterization of a temperature-sensitive dnaK mutant of Escherichia coli B. J. Bacteriol. 138:339-44 |
|---|---|
| Abstract |
A temperature-sensitive dnaK mutant (strain MT112) was isolated from Escherichia coli B strain H/r30RT by thymineless death selection at 43 degrees C. By genetic mapping, the mutation [dnaK7(Ts)] was located near the thr gene (approximately 0.2 min on the may). E. coli K-12 transductants of the mutation to temperature sensitivity were assayed for their susceptibility to transducing phage lambda carrying the dnaK and/or the dnaJ gene. All of the transductants were able to propagate phage lambda carrying the dnaK gene. When macromolecular synthesis of the mutant was assayed at 43 degrees C, it was observed that both deoxyribonucleic acid and ribonucleic acid syntheses were severely inhibited. Thus, it was suggested that the conditionally defective dnaK mutation affects both cellular deoxyribonucleic acid and ribonucleic acid syntheses at the nonpermissive temperature in addition to inability to propagate phage lambda at permissive temperature. |
| Links | |
| Keywords |
Chromosome Mapping; Coliphages/genetics; DNA, Bacterial/biosynthesis; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Lysogeny; Mutation; RNA, Bacterial/biosynthesis; Temperature; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
Notes
References
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