Difference between revisions of "PMID:18067575"

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'''Lee, Y, Kim, Y, Yeom, S, Kim, S, Park, S, Jeon, CO and Park, W'''  (2008) The role of disulfide bond isomerase A (DsbA) of Escherichia coli O157:H7 in biofilm formation and virulence. ''FEMS Microbiol. Lett.'' '''278''':213-22
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!align=left align='left' bgcolor='#CCCCFF' |Abstract
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The role of periplasmic disulfide oxidoreductase DsbA in Shiga toxin-producing Escherichia coli O157:H7 (STEC) was investigated. Deletion of dsbA (DeltadsbA) significantly decreased cell motility and alkaline phosphatase activity in STEC. STEC DeltadsbA also showed greater sensitivity to menadione and under low pH conditions. Significant reductions in surface attachment to both biotic (HT-29 epithelial cells) and abiotic (polystyrene and polyvinyl chloride) surfaces were observed in STEC DeltadsbA. In addition, no biofilm formation was detected in STEC DeltadsbA compared to wild-type cells in glass capillary tubes under continuous flow-culture system conditions. In the nematode model Caenorhabditis elegans-killing assay, the deletion of dsbA in STEC resulted in attenuated virulence compared to wild-type cells. STEC DeltadsbA was also found to have a reduced ability to colonize the nematode gut. These results suggest that DsbA plays important roles in biofilm formation and virulence in STEC cells.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18067575 PubMed]
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Online version:[http://dx.doi.org/10.1111/j.1574-6968.2007.00993.x 10.1111/j.1574-6968.2007.00993.x]
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!align=left align='left' bgcolor='#CCCCFF' |Keywords
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Alkaline Phosphatase/metabolism; Animals; Bacterial Adhesion/genetics; Bacterial Adhesion/physiology; Biofilms/growth & development; Blotting, Northern; Caenorhabditis elegans/microbiology; Cell Line, Tumor; Escherichia coli O157/drug effects; Escherichia coli O157/genetics; Escherichia coli O157/pathogenicity; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Gene Expression Regulation, Bacterial; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Humans; Hydrogen-Ion Concentration; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Models, Genetic; Mutation; Protein Disulfide-Isomerases/genetics; Protein Disulfide-Isomerases/metabolism; Protein Disulfide-Isomerases/physiology; Virulence/genetics; Vitamin K 3/pharmacology
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="D5c7e5f08b65c4"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 06:35, 5 March 2019

Citation

Lee, Y, Kim, Y, Yeom, S, Kim, S, Park, S, Jeon, CO and Park, W (2008) The role of disulfide bond isomerase A (DsbA) of Escherichia coli O157:H7 in biofilm formation and virulence. FEMS Microbiol. Lett. 278:213-22

Abstract

The role of periplasmic disulfide oxidoreductase DsbA in Shiga toxin-producing Escherichia coli O157:H7 (STEC) was investigated. Deletion of dsbA (DeltadsbA) significantly decreased cell motility and alkaline phosphatase activity in STEC. STEC DeltadsbA also showed greater sensitivity to menadione and under low pH conditions. Significant reductions in surface attachment to both biotic (HT-29 epithelial cells) and abiotic (polystyrene and polyvinyl chloride) surfaces were observed in STEC DeltadsbA. In addition, no biofilm formation was detected in STEC DeltadsbA compared to wild-type cells in glass capillary tubes under continuous flow-culture system conditions. In the nematode model Caenorhabditis elegans-killing assay, the deletion of dsbA in STEC resulted in attenuated virulence compared to wild-type cells. STEC DeltadsbA was also found to have a reduced ability to colonize the nematode gut. These results suggest that DsbA plays important roles in biofilm formation and virulence in STEC cells.

Links

PubMed Online version:10.1111/j.1574-6968.2007.00993.x

Keywords

Alkaline Phosphatase/metabolism; Animals; Bacterial Adhesion/genetics; Bacterial Adhesion/physiology; Biofilms/growth & development; Blotting, Northern; Caenorhabditis elegans/microbiology; Cell Line, Tumor; Escherichia coli O157/drug effects; Escherichia coli O157/genetics; Escherichia coli O157/pathogenicity; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Gene Expression Regulation, Bacterial; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Humans; Hydrogen-Ion Concentration; Luminescent Proteins/genetics; Luminescent Proteins/metabolism; Models, Genetic; Mutation; Protein Disulfide-Isomerases/genetics; Protein Disulfide-Isomerases/metabolism; Protein Disulfide-Isomerases/physiology; Virulence/genetics; Vitamin K 3/pharmacology

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Main Points of the Paper

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Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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