Difference between revisions of "Category:ECO:0000226 ! chromatin immunoprecipitation evidence"
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'''def:''' "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]<br> | '''def:''' "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]<br> | ||
'''synonym:''' "ChIP evidence" EXACT []<br> | '''synonym:''' "ChIP evidence" EXACT []<br> | ||
| − | '''xref:''' [ | + | '''xref:''' [ MI:0402 "chromatin immunoprecipitation assay"]<br> |
'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]]<br> | '''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]]<br> | ||
'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br> | '''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br> | ||
Revision as of 17:59, 24 October 2017
| id: ECO:0000226 name: chromatin immunoprecipitation evidence |
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