Difference between revisions of "Category:Motility Assay"
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== Methods == | == Methods == | ||
Semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/> | Semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/> | ||
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| + | Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h." | ||
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| + | Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/> | ||
== Examples == | == Examples == | ||
Revision as of 11:47, 6 June 2011
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Purpose
Technical Details
Materials Needed
Methods
Semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. [1]
Motility assay after Sperandio et al.[2]: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h."
Modified motility assay, adapted from Sperandio et al.[2]: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.[3]
Examples
Notes
References
See Help:References for how to manage references in OMPwiki.
- ↑ Rajagopala, SV et al. (2007) The protein network of bacterial motility. Mol. Syst. Biol. 3 128 PubMed OMPwiki page
- ↑ 2.0 2.1 Sperandio, V et al. (2002) Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol. Microbiol. 43 809-21 PubMed OMPwiki page
- ↑ Herzberg, M et al. (2006) YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport. J. Bacteriol. 188 587-98 PubMed OMPwiki page
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