Difference between revisions of "Category:Motility Assay"
(→Examples) |
m |
||
| (6 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
{{Pagetop}} | {{Pagetop}} | ||
== Purpose == | == Purpose == | ||
| + | Motility assays measure the ability of cells to perform directed movement. | ||
| − | == | + | ==Papers that describe swimming assays== |
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h." | Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h." | ||
Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/> | Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/> | ||
| + | |||
| + | ==Papers that describe swarming assays== | ||
| + | Rajagopala et al. describe a semi-high-throughput motility (swarming) assay, using 24 colonies per plate on microtiter-sized Omnitrays (Nunc) with swarming agar (LB medium with 0.2% Agar) .<ref name='PMID: 17667950'/> | ||
== Notes == | == Notes == | ||
Latest revision as of 12:30, 17 May 2017
You can help OMP wiki by editing the content of this page. For information about becoming a registered user and obtaining editing privileges, see Help:Accounts.
Contents
Purpose
Motility assays measure the ability of cells to perform directed movement.
Papers that describe swimming assays
Motility assay after Sperandio et al.[1]: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h."
Modified motility assay, adapted from Sperandio et al.[1]: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.[2]
Papers that describe swarming assays
Rajagopala et al. describe a semi-high-throughput motility (swarming) assay, using 24 colonies per plate on microtiter-sized Omnitrays (Nunc) with swarming agar (LB medium with 0.2% Agar) .[3]
Notes
References
See Help:References for how to manage references in OMPwiki.
- ↑ 1.0 1.1 Sperandio, V et al. (2002) Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol. Microbiol. 43 809-21 PubMed OMPwiki page
- ↑ Herzberg, M et al. (2006) YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport. J. Bacteriol. 188 587-98 PubMed OMPwiki page
- ↑ Rajagopala, SV et al. (2007) The protein network of bacterial motility. Mol. Syst. Biol. 3 128 PubMed OMPwiki page
This category currently contains no pages or media.
