Category:Motility Assay - Revision history

Siegele at 17:30, 17 May 2017

2017-05-17T17:30:05Z

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	== Purpose ==		== Purpose ==
	Motility assays measure the ability of cells to perform directed movement.		Motility assays measure the ability of cells to perform directed movement.


	==Papers that describe swimming assays==		==Papers that describe swimming assays==
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	==Papers that describe swarming assays==		==Papers that describe swarming assays==
	Rajagopala et al. describe a semi-high-throughput motility (swarming) assay, using 24 colonies per plate on microtiter-sized Omnitrays (Nunc) with swarming agar (LB medium with 0.2% Agar) . <ref name='PMID: 17667950'/>		Rajagopala et al. describe a semi-high-throughput motility (swarming) assay, using 24 colonies per plate on microtiter-sized Omnitrays (Nunc) with swarming agar (LB medium with 0.2% Agar) .<ref name='PMID: 17667950'/>

	== Notes ==		== Notes ==

Siegele: /* Papers that describe swarming assays */

2017-05-17T17:29:37Z

Papers that describe swarming assays

← Older revision		Revision as of 17:29, 17 May 2017
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	==Papers that describe swarming assays==		==Papers that describe swarming assays==
	Rajagopala et al. describe a semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized ~~omnitrays~~. <ref name='PMID: 17667950'/>		Rajagopala et al. describe a semi-high-throughput motility (swarming) assay, using 24 colonies per plate on microtiter-sized Omnitrays (Nunc) with swarming agar (LB medium with 0.2% Agar) . <ref name='PMID: 17667950'/>

	== Notes ==		== Notes ==

Siegele at 17:26, 17 May 2017

2017-05-17T17:26:45Z

← Older revision		Revision as of 17:26, 17 May 2017
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	{{Pagetop}}		{{Pagetop}}
	== Purpose ==		== Purpose ==
	Motility assays measure the ability of cells to ~~move around. Hence such assays usually measure the activity of flagella~~.		Motility assays measure the ability of cells to perform directed movement.

	~~== Technical Details ==~~

	~~== Materials Needed ==~~

	~~== Methods ==~~
	~~Rajagopala et al. describe a semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/>~~

			==Papers that describe swimming assays==
	Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h."		Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h."

	Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/>		Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/>

	== ~~Examples~~ ==		==Papers that describe swarming assays==
			Rajagopala et al. describe a semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/>

	== Notes ==		== Notes ==

Puetz: /* Purpose */

2011-06-06T16:52:16Z

Purpose

← Older revision		Revision as of 16:52, 6 June 2011
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	{{Pagetop}}		{{Pagetop}}
	== Purpose ==		== Purpose ==
			Motility assays measure the ability of cells to move around. Hence such assays usually measure the activity of flagella.

	== Technical Details ==		== Technical Details ==

Puetz: /* Methods */

2011-06-06T16:48:08Z

Methods

← Older revision		Revision as of 16:48, 6 June 2011
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	== Methods ==		== Methods ==
	~~Semi~~-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/>		Rajagopala et al. describe a semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/>

	Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h."		Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h."

Puetz: /* Methods */

2011-06-06T16:47:39Z

Methods

← Older revision		Revision as of 16:47, 6 June 2011
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	== Methods ==		== Methods ==
	Semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/>		Semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/>

			Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h."

			Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/>

	== Examples ==		== Examples ==

Puetz: /* Examples */

2011-06-06T16:47:29Z

Examples

← Older revision		Revision as of 16:47, 6 June 2011
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	== Examples ==		== Examples ==
	Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h."

	Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/>

	== Notes ==		== Notes ==

Puetz: /* Examples */

2011-06-06T16:45:23Z

Examples

← Older revision		Revision as of 16:45, 6 June 2011
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	== Examples ==		== Examples ==
	Motility ~~assays were performed by~~ Sperandio et al.<ref name='PMID: 11929534'/> at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h.		Motility assay after Sperandio et al.<ref name='PMID: 11929534'/>: cells were grown "at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h."

	Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/>		Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/>

Puetz: /* Examples */

2011-06-06T16:44:03Z

Examples

← Older revision		Revision as of 16:44, 6 June 2011
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	== Examples ==		== Examples ==
	Motility assays were performed by Sperandio et al.<ref name='PMID: 11929534'/> at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h.		Motility assays were performed by Sperandio et al.<ref name='PMID: 11929534'/> at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h.

			Modified motility assay, adapted from Sperandio et al.<ref name='PMID: 11929534'/>: LB overnight cultures were used to assay motility in plates containing 1% (wt/vol) tryptone, 0.25% (wt/vol) NaCl, and 0.3% (wt/vol) agar (where indicated, motility plates were supplemented with glucose or chloramphenicol). Motility halos were measured at 16 h, and five to eight plates were used to compare motility between the strains. For the motility complementation experiment, motility ratios be- tween the ydgG mutant and the wild-type strain are used in order to eliminate the small density differences between the motility plates. On each plate, both the wild type and the ydgG mutant were inoculated.<ref name='PMID: 16385049'/>

	== Notes ==		== Notes ==

Puetz at 16:39, 6 June 2011

2011-06-06T16:39:04Z

← Older revision		Revision as of 16:39, 6 June 2011
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	== Methods ==		== Methods ==
			Semi-high-throughput motility (swarming) assay, using 24 colonies on microtiter-sized omnitrays. <ref name='PMID: 17667950'/>

			== Examples ==
			Motility assays were performed by Sperandio et al.<ref name='PMID: 11929534'/> at 37C on 0.3% agar plates containing: DMEM, DMEM + 50% DMEM preconditioned by growth of EHEC 86-24 or tryptone media (1% tryptone and 0.25% NaCl). The motility halos were measured at 8, 16 and 48 h.

	== Notes ==		== Notes ==

	~~== Related Ontology Terms ==~~

	== References ==		== References ==