https://microbialphenotypes.org/wiki/index.php?action=history&feed=atom&title=Category%3AMacConkey_Agar 2026-10-24T18:14:50Z Revision history for this page on the wiki MediaWiki 1.31.1 https://microbialphenotypes.org/wiki/index.php?title=Category:MacConkey_Agar&diff=2483&oldid=prev 2011-04-06T21:29:03Z

<p></p> <table class="diff diff-contentalign-left" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 21:29, 6 April 2011</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l26" >Line 26:</td> <td colspan="2" class="diff-lineno">Line 26:</td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>&lt;references/&gt;</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>&lt;references/&gt;</div></td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'>&#160;</td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del class="diffchange diffchange-inline">[[Category:Methods]]</del>[[Category:Growth Medium]][[Category:Indicator Medium]][[Category:Selective Medium]]</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[Category:Growth Medium]][[Category:Indicator Medium]][[Category:Selective Medium]]</div></td></tr> </table>

Azweifel https://microbialphenotypes.org/wiki/index.php?title=Category:MacConkey_Agar&diff=2078&oldid=prev 2011-03-23T01:38:09Z

<p>making into a CategoryPage</p> <p><b>New page</b></p><div>{{Pagetop}}<br /> == Purpose ==<br /> MacConkey agar is a differential fermentation indicator used to determine whether bacteria can metabolize sugars, commonly lactose.&lt;ref name='PMID:20474229'/&gt; It also acts as a selective media because the bile salts and crystal violet present prevent Gram-positive bacteria from forming colonies.<br /> <br /> == Technical Details ==<br /> Bacteria grown on this medium may either use peptone, or the designated sugar as its energy source. Bacteria that can metabolize the sugar produce acid that turn the colonies red. The acid is also secreted into the medium, lowering the pH around the colony. In response to the lowered pH, bile salts in the agar precipitate, causing the surrounding medium to become opaque.&lt;ref name='PMID:20474363'/&gt; Bacteria that cannot metabolize the sugar will utilize peptone instead; the ammonia that results will rai se the pH of the surrounding medium, leading to white colonies and transparent agar.<br /> <br /> == Materials Needed ==<br /> *MacConkey base<br /> *lactose or other fermentable sugar<br /> *distilled water<br /> <br /> == Methods ==<br /> <br /> == Examples ==<br /> *Colonies that are able to metabolize the added sugar appear dark red while those that cannot appear white. There are intermediate levels of color (e.g. light red) which coincide with the rate or level of fermentation.<br /> *Improved specificity identifying ''Shigella''&lt;ref name='PMID:8724396'/&gt;<br /> *[http://www.microbelibrary.org/index.php/component/resource/laboratory-test/2850-macconkey-agar-plates Microbe Library MacConkey agar sample plates]<br /> <br /> == Notes ==<br /> *Red Colonies: ''E. coli'', ''Enterobacter'', and ''Klebsiella'' <br /> *White Colonies: ''Salmonella'', ''Proteus'', ''P. aeruginosa'', and ''Shigella''<br /> <br /> == References ==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> [[Category:Methods]][[Category:Growth Medium]][[Category:Indicator Medium]][[Category:Selective Medium]]</div>

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