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== Purpose ==<br />
*Suppression of mutants defective in initiation of DNA replication due to a mutation in ''dnaA''<ref name='PMID:4927942'/><ref name='PMID:4599365'/><br />
*Isolation of mutants defective in initiation of DNA replication<ref name='PMID:7007354'/><br />
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== Technical Details ==<br />
*To suppress ''dnaA''-based initiation of DNA replication mutants<br />
At restrictive temperature, the DnaA mutant protein does not allow for initiation of DNA replication at ''oriC''. This defect is suppressed by initiation from the integrated plasmid's origin through which replication of the chromosome proceeds. This mechanism results in temperature-resistant, DnaA-independent initiation of DNA replication.<ref name='PMID:4927942'/><ref name='PMID:4599365'/><br />
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== Materials Needed ==<br />
*Various replicons can be used<br />
**F and F' factors<ref name='PMID:4927942'/><br />
**R factors<ref name='PMID:4584797'/><br />
**bacteriophage P1 and P7<ref name='PMID:372960'/><br />
**bacteriophage P2 ''sig5'' (mutant bacteriophage of P2)<ref name='PMID:350855'/><ref name='PMID:4944622'/><br />
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== Methods ==<br />
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== Examples ==<br />
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== Notes ==<br />
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== References ==<br />
{{RefHelp}}<br />
<references/><br />
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