Azweifel at 21:54, 6 April 2011

2011-04-06T21:54:36Z

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2011-03-23T01:38:06Z

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{{Pagetop}}
== Purpose ==
*To recover gastrointestinal pathogens, such as ''Salmonella'' and ''Shigella'', from clinical specimens<ref name='PMID:4869617'/><ref name='PMID:5647515'/>
*Inhibits most nonpathogenic enteric organisms

== Technical Details ==
*The presence of bile salts and dye inhibit the growth of most Gram-positive bacteria
*Lactose, sucrose, and salicin are carbohydrates in the medium
**Strains (generally nonpathogenic) that are able to utilize any of these sugars appear as orange-yellow colonies
**Strains that strictly utilize salicin will appear salmon-pink to orange-yellow
**Strains unable to utilize any of these and will appear as translucent, light green, or blueish-green (i.e. ''Salmonella'' and ''Shigella'')
*The addition of thiosulfate and ferric ammonium citrate can detect the production of H<sub>2</sub>S
**Colonies appear black or have black centers (e.g. ''Salmonella'')<ref>Winn, W., Jr., et al. 2006. Konemann’s color atlas and diagnostic text of microbiology, 6th ed., p. 212–302. Lippencott Williams & Wilkins Publishers, Philadelphia, PA.</ref>

== Materials Needed ==
*Pre-mixed powder is available for purchase. The components are listed below.<ref>Difco. 1984. Difco manual, 10th ed. Difco Laboratories, Detroit, MI.</ref>
**Proteose, peptone
**Yeast extract
**Bile salts no.3
**Lactose
**Saccharose
**Salicin
**Sodium chloride
**Sodium thiosulfate
**Ferric ammonium citrate
**Acid fuchsin
**Bromthymol blue
**Agar

== Methods ==
*Specific information on preparing these plates can be found [http://www.microbelibrary.org/index.php/library/laboratory-test/3222-hektoen-enteric-agar-protocol here].

== Examples ==
*[http://www.microbelibrary.org/index.php/library/laboratory-test/3276-hektoen-enteric-agar Microbe Library Hektoen Enteric Agar sample plates]

== Notes ==
*''Salmonella'' serovar Typhi is a weak H<sub>2</sub>S producer, so may not appear black
*Hektoen Agar was named after the institute where studies were conducted<ref name='PMID:4869617'/>

== References ==
{{RefHelp}}
<references/>

[[Category:Methods]][[Category:Growth Medium]][[Category:Indicator Medium]][[Category:Selective Medium]]

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Category:Hektoen Enteric Agar - Revision history

Azweifel at 21:54, 6 April 2011

127.0.0.1: making into a CategoryPage