Category:ECO:0000295 ! RNA-seq evidence - Revision history

127.0.0.1: update ECO:0000295_!_RNA-seq_evidence page

2019-04-08T11:05:23Z

update ECO:0000295_!_RNA-seq_evidence page

127.0.0.1: ECO:0000295_!_RNA-seq_evidence renamed to ECO:0000295_!_RNA-sequencing_evidence

2019-01-06T08:01:19Z

ECO:0000295_!_RNA-seq_evidence renamed to ECO:0000295_!_RNA-sequencing_evidence

127.0.0.1: update ECO:0000295_!_RNA-seq_evidence page

2018-06-16T07:01:00Z

update ECO:0000295_!_RNA-seq_evidence page

127.0.0.1: update ECO:0000295_!_RNA-seq_evidence page

2018-03-04T08:00:39Z

update ECO:0000295_!_RNA-seq_evidence page

127.0.0.1: update ECO:0000295_!_RNA-seq_evidence page

2017-10-24T22:59:14Z

update ECO:0000295_!_RNA-seq_evidence page

127.0.0.1: Add page for ECO:0000295_!_RNA-seq_evidence

2013-03-05T20:52:21Z

Add page for ECO:0000295_!_RNA-seq_evidence

New page

{{Autobox|1='''id:''' ECO:0000295 
'''name:''' RNA-seq evidence 
'''def:''' "A type of transcript expression evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] 
'''synonym:''' "RNA sequencing" RELATED [] 
'''synonym:''' "whole transcriptome shotgun sequencing" EXACT [PMID:18611170] 
'''synonym:''' "WTSS" EXACT [] 
'''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]] 
'''comment:''' Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed. 
}}
== Usage Notes ==

==References ==
{{RefHelp}}
<references/>

← Older revision		Revision as of 11:05, 8 April 2019
Line 1:		Line 1:
−	~~#REDIRECT~~ [[:Category:ECO:~~0000295_~~!~~_RNA-sequencing_evidence~~]]	+	{{Autobox\|1='''id:''' ECO:0000295<br>
		+	'''name:''' RNA-seq evidence<br>
		+	'''def:''' "A type of transcript evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC]<br>
		+	'''alt_id:''' ECO:0000357<br>
		+	'''synonym:''' "differential gene expression evidence from RNA-seq experiment" NARROW []<br>
		+	'''synonym:''' "RNA sequencing" RELATED []<br>
		+	'''synonym:''' "whole transcriptome shotgun sequencing" EXACT [PMID:18611170]<br>
		+	'''synonym:''' "WTSS" EXACT []<br>
		+	'''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence\|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence\|ECO:0000009 ! transcript expression evidence]]<br>
		+	'''relationship:''' IAO:0000136: [http://gowiki.tamu.edu/index.php/Category:GO:0010467_!_is_about_gene_expression GO:0010467 ! is about gene expression]<br>
		+	'''relationship:''' OBI:0000312: OBI:0001271 ! is_specified_output_of RNA-seq assay<br>
		+	'''comment:''' Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed.<br>
		+	}}
	== Usage Notes ==		== Usage Notes ==

← Older revision		Revision as of 08:01, 6 January 2019
Line 1:		Line 1:
−	~~{{Autobox\|1='''id:''' ECO:0000295<br>~~	+	#REDIRECT [[:Category:ECO:0000295_!_RNA-sequencing_evidence]]
−	~~'''name:''' RNA-seq evidence<br>~~
−	~~'''def:''' "A type of transcript evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC]<br>~~
−	~~'''alt_id:''' ECO:0000357<br>~~
−	~~'''synonym:''' "differential gene expression evidence from RNA-seq experiment" NARROW []<br>~~
−	~~'''synonym:''' "RNA sequencing" RELATED []<br>~~
−	~~'''synonym:''' "whole transcriptome shotgun sequencing" EXACT [PMID:18611170]<br>~~
−	~~'''synonym:''' "WTSS" EXACT []<br>~~
−	~~'''is_a:'''~~ [[:Category:ECO:~~0000009~~ ! ~~transcript expression evidence\|ECO:0000009 ! transcript expression evidence~~]]~~[[Category:ECO:0000009 ! transcript expression evidence\|ECO:0000009 ! transcript expression evidence]]<br>~~
−	~~'''relationship:''' IAO:0000136: [http://gowiki.tamu.edu/index.php/Category:GO:0010467_!_is_about_gene_expression GO:0010467 ! is about gene expression]<br>~~
−	~~'''relationship:''' OBI:0000312: OBI:0001271 ! is_specified_output_of RNA-seq assay<br>~~
−	'''comment:''' Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed.<br>
−	}}
	== Usage Notes ==		== Usage Notes ==

@@ Line 8: / Line 8: @@
 '''synonym:''' "WTSS" EXACT []<br>
 '''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]]<br>
 '''comment:''' Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed.<br>
 }}

@@ Line 2: / Line 2: @@
 '''name:''' RNA-seq evidence<br>
 '''def:''' "A type of transcript evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC]<br>
 '''synonym:''' "RNA sequencing" RELATED []<br>
 '''synonym:''' "whole transcriptome shotgun sequencing" EXACT [PMID:18611170]<br>

@@ Line 1: / Line 1: @@
 {{Autobox|1='''id:''' ECO:0000295<br>
 '''name:''' RNA-seq evidence<br>
-'''def:''' "A type of transcript expression evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC]<br>
+'''def:''' "A type of transcript evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC]<br>
 '''synonym:''' "RNA sequencing" RELATED []<br>
 '''synonym:''' "whole transcriptome shotgun sequencing" EXACT [PMID:18611170]<br>