Category:ECO:0000226 ! chromatin immunoprecipitation evidence - Revision history

127.0.0.1: update ECO:0000226_!_chromatin_immunoprecipitation_evidence page

2018-06-16T07:01:15Z

update ECO:0000226_!_chromatin_immunoprecipitation_evidence page

← Older revision		Revision as of 07:01, 16 June 2018
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	'''xref:''' [ MI:0402 "chromatin immunoprecipitation assay"]<br>		'''xref:''' [ MI:0402 "chromatin immunoprecipitation assay"]<br>
	'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]]<br>		'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]]<br>
			'''relationship:''' IAO:0000136: [http://gowiki.tamu.edu/index.php/Category:GO:0043565_!_is_about_sequence-specific_DNA_binding GO:0043565 ! is about sequence-specific DNA binding]<br>
			'''relationship:''' OBI:0000312: OBI:0001956 ! is_specified_output_of assay using chromatin immunoprecipitation<br>
	'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>		'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>
	}}		}}

127.0.0.1: update ECO:0000226_!_chromatin_immunoprecipitation_evidence page

2017-10-24T22:59:16Z

update ECO:0000226_!_chromatin_immunoprecipitation_evidence page

← Older revision		Revision as of 22:59, 24 October 2017
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	'''def:''' "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]<br>		'''def:''' "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]<br>
	'''synonym:''' "ChIP evidence" EXACT []<br>		'''synonym:''' "ChIP evidence" EXACT []<br>
	'''xref:''' [~~http://psidev.sourceforge.net/mi/xml/doc/user/index.html PSI-~~MI~~:MI\~~:0402 "chromatin immunoprecipitation assay"]<br>		'''xref:''' [ MI:0402 "chromatin immunoprecipitation assay"]<br>
	'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]]<br>		'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]]<br>
	'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>		'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>

127.0.0.1: update ECO:0000226_!_chromatin_immunoprecipitation_evidence page

2013-04-23T18:49:18Z

update ECO:0000226_!_chromatin_immunoprecipitation_evidence page

← Older revision		Revision as of 18:49, 23 April 2013
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	'''def:''' "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]<br>		'''def:''' "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]<br>
	'''synonym:''' "ChIP evidence" EXACT []<br>		'''synonym:''' "ChIP evidence" EXACT []<br>
	'''xref:''' [ PSI-MI:MI\:0402 "chromatin immunoprecipitation assay"]<br>		'''xref:''' [http://psidev.sourceforge.net/mi/xml/doc/user/index.html PSI-MI:MI\:0402 "chromatin immunoprecipitation assay"]<br>
	'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]]<br>		'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]]<br>
	'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>		'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>

127.0.0.1: update ECO:0000226_!_chromatin_immunoprecipitation_evidence page

2013-03-19T06:00:08Z

update ECO:0000226_!_chromatin_immunoprecipitation_evidence page

← Older revision		Revision as of 06:00, 19 March 2013
Line 3:		Line 3:
	'''def:''' "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]<br>		'''def:''' "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]<br>
	'''synonym:''' "ChIP evidence" EXACT []<br>		'''synonym:''' "ChIP evidence" EXACT []<br>
	'''xref:''' [~~http://psidev.sourceforge.net/mi/xml/doc/user/index.html~~ PSI-MI:MI\:0402 "chromatin immunoprecipitation assay"]<br>		'''xref:''' [ PSI-MI:MI\:0402 "chromatin immunoprecipitation assay"]<br>
	'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]]<br>		'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence\|ECO:0000085 ! immunoprecipitation evidence]]<br>
	'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>		'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).<br>

127.0.0.1: Add page for ECO:0000226_!_chromatin_immunoprecipitation_evidence

2013-03-05T20:52:13Z

Add page for ECO:0000226_!_chromatin_immunoprecipitation_evidence

New page

{{Autobox|1='''id:''' ECO:0000226 
'''name:''' chromatin immunoprecipitation evidence 
'''def:''' "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC] 
'''synonym:''' "ChIP evidence" EXACT [] 
'''xref:''' [http://psidev.sourceforge.net/mi/xml/doc/user/index.html PSI-MI:MI\:0402 "chromatin immunoprecipitation assay"] 
'''is_a:''' [[:Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]][[Category:ECO:0000085 ! immunoprecipitation evidence|ECO:0000085 ! immunoprecipitation evidence]] 
'''comment:''' Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq). 
}}
== Usage Notes ==

==References ==
{{RefHelp}}
<references/>