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Category:ECO:0000223 ! 454 pyrosequencing evidence - Revision history
2026-11-03T18:05:17Z
Revision history for this page on the wiki
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127.0.0.1: update ECO:0000223_!_454_pyrosequencing_evidence page
2017-12-04T08:00:49Z
<p>update ECO:0000223_!_454_pyrosequencing_evidence page</p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 08:00, 4 December 2017</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Autobox|1='''id:''' ECO:0000223<br></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Autobox|1='''id:''' ECO:0000223<br></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''name:''' 454 pyrosequencing evidence<br></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''name:''' 454 pyrosequencing evidence<br></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>'''def:''' "A high throughput nucleotide sequencing <del style="font-weight: bold; text-decoration: none;">result derived </del>by <del style="font-weight: bold; text-decoration: none;">454 pyrosequencing technology</del>." [<del style="font-weight: bold; text-decoration: none;">ECO</del>:<del style="font-weight: bold; text-decoration: none;">MCC</del>]<br></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>'''def:''' "A <ins style="font-weight: bold; text-decoration: none;">type of </ins>high throughput nucleotide sequencing <ins style="font-weight: bold; text-decoration: none;">evidence in which the sequence for a ssDNA is determined </ins>by <ins style="font-weight: bold; text-decoration: none;">addition of primers and then nucleotides, one base pair at a time (which generate a specific fluorescence), to the target strand</ins>." [<ins style="font-weight: bold; text-decoration: none;">EFO</ins>:<ins style="font-weight: bold; text-decoration: none;">0005016</ins>]<br></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''synonym:''' "pyrosequencing" BROAD [OBI:0000730]<br></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''synonym:''' "pyrosequencing" BROAD [OBI:0000730]<br></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''is_a:''' [[:Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]][[Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]]<br></div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''is_a:''' [[:Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]][[Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]]<br></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>'''comment:''' 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.<br></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>'''comment:''' 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA<ins style="font-weight: bold; text-decoration: none;">.\n\nThe target strand is usually prepared by fragmentation, adaptor ligation, and amplification by emulsion PCR</ins>.<br></div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Usage Notes ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Usage Notes ==</div></td></tr>
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127.0.0.1
https://microbialphenotypes.org/wiki/index.php?title=Category:ECO:0000223_!_454_pyrosequencing_evidence&diff=9832&oldid=prev
127.0.0.1: Add page for ECO:0000223_!_454_pyrosequencing_evidence
2013-03-05T20:52:12Z
<p>Add page for ECO:0000223_!_454_pyrosequencing_evidence</p>
<p><b>New page</b></p><div>{{Autobox|1='''id:''' ECO:0000223<br><br />
'''name:''' 454 pyrosequencing evidence<br><br />
'''def:''' "A high throughput nucleotide sequencing result derived by 454 pyrosequencing technology." [ECO:MCC]<br><br />
'''synonym:''' "pyrosequencing" BROAD [OBI:0000730]<br><br />
'''is_a:''' [[:Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]][[Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]]<br><br />
'''comment:''' 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.<br><br />
}}<br />
== Usage Notes ==<br />
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==References ==<br />
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127.0.0.1