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	<id>https://microbialphenotypes.org/wiki/index.php?action=history&amp;feed=atom&amp;title=Category%3AECO%3A0000222_%21_Illumina_sequencing_evidence</id>
	<title>Category:ECO:0000222 ! Illumina sequencing evidence - Revision history</title>
	<link rel="self" type="application/atom+xml" href="https://microbialphenotypes.org/wiki/index.php?action=history&amp;feed=atom&amp;title=Category%3AECO%3A0000222_%21_Illumina_sequencing_evidence"/>
	<link rel="alternate" type="text/html" href="https://microbialphenotypes.org/wiki/index.php?title=Category:ECO:0000222_!_Illumina_sequencing_evidence&amp;action=history"/>
	<updated>2026-11-03T10:47:40Z</updated>
	<subtitle>Revision history for this page on the wiki</subtitle>
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	<entry>
		<id>https://microbialphenotypes.org/wiki/index.php?title=Category:ECO:0000222_!_Illumina_sequencing_evidence&amp;diff=66412&amp;oldid=prev</id>
		<title>127.0.0.1: update ECO:0000222_!_Illumina_sequencing_evidence page</title>
		<link rel="alternate" type="text/html" href="https://microbialphenotypes.org/wiki/index.php?title=Category:ECO:0000222_!_Illumina_sequencing_evidence&amp;diff=66412&amp;oldid=prev"/>
		<updated>2017-12-04T08:00:49Z</updated>

		<summary type="html">&lt;p&gt;update ECO:0000222_!_Illumina_sequencing_evidence page&lt;/p&gt;
&lt;table style=&quot;background-color: #fff; color: #202122;&quot; data-mw=&quot;interface&quot;&gt;
				&lt;col class=&quot;diff-marker&quot; /&gt;
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				&lt;td colspan=&quot;2&quot; style=&quot;background-color: #fff; color: #202122; text-align: center;&quot;&gt;← Older revision&lt;/td&gt;
				&lt;td colspan=&quot;2&quot; style=&quot;background-color: #fff; color: #202122; text-align: center;&quot;&gt;Revision as of 08:00, 4 December 2017&lt;/td&gt;
				&lt;/tr&gt;&lt;tr&gt;&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot; id=&quot;mw-diff-left-l1&quot;&gt;Line 1:&lt;/td&gt;
&lt;td colspan=&quot;2&quot; class=&quot;diff-lineno&quot;&gt;Line 1:&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;{{Autobox|1=&amp;#039;&amp;#039;&amp;#039;id:&amp;#039;&amp;#039;&amp;#039; ECO:0000222&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;{{Autobox|1=&amp;#039;&amp;#039;&amp;#039;id:&amp;#039;&amp;#039;&amp;#039; ECO:0000222&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;&amp;#039;&amp;#039;&amp;#039;name:&amp;#039;&amp;#039;&amp;#039; Illumina sequencing evidence&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;&amp;#039;&amp;#039;&amp;#039;name:&amp;#039;&amp;#039;&amp;#039; Illumina sequencing evidence&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot; data-marker=&quot;−&quot;&gt;&lt;/td&gt;&lt;td style=&quot;color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;&#039;&#039;&#039;def:&#039;&#039;&#039; &quot;A high throughput nucleotide sequencing &lt;del style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;result derived &lt;/del&gt;by &lt;del style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;Illumina (Solexa) sequencing technology&lt;/del&gt;.&quot; [&lt;del style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;ECO&lt;/del&gt;:&lt;del style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;MCC&lt;/del&gt;]&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot; data-marker=&quot;+&quot;&gt;&lt;/td&gt;&lt;td style=&quot;color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;&#039;&#039;&#039;def:&#039;&#039;&#039; &quot;A &lt;ins style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;type of &lt;/ins&gt;high throughput nucleotide sequencing &lt;ins style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;evidence in which sequence is determined through hybridization of adaptor-ligated DNA to a glass slide on which each fragment is simultaneously clonally amplified, followed &lt;/ins&gt;by &lt;ins style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;progressive rounds of base incorporation with fluorescently-tagged nucleotides, washing, imaging, and cleavage&lt;/ins&gt;.&quot; [&lt;ins style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;OBI:0000724, PMID&lt;/ins&gt;:&lt;ins style=&quot;font-weight: bold; text-decoration: none;&quot;&gt;26000844&lt;/ins&gt;]&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;&amp;#039;&amp;#039;&amp;#039;synonym:&amp;#039;&amp;#039;&amp;#039; &amp;quot;Solexa sequencing result&amp;quot; RELATED [OBI:0000724]&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;&amp;#039;&amp;#039;&amp;#039;synonym:&amp;#039;&amp;#039;&amp;#039; &amp;quot;Solexa sequencing result&amp;quot; RELATED [OBI:0000724]&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;tr&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;&amp;#039;&amp;#039;&amp;#039;is_a:&amp;#039;&amp;#039;&amp;#039; [[:Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]][[Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]]&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;td class=&quot;diff-marker&quot;&gt;&lt;/td&gt;&lt;td style=&quot;background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;&quot;&gt;&lt;div&gt;&amp;#039;&amp;#039;&amp;#039;is_a:&amp;#039;&amp;#039;&amp;#039; [[:Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]][[Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]]&amp;lt;br&amp;gt;&lt;/div&gt;&lt;/td&gt;&lt;/tr&gt;
&lt;/table&gt;</summary>
		<author><name>127.0.0.1</name></author>
	</entry>
	<entry>
		<id>https://microbialphenotypes.org/wiki/index.php?title=Category:ECO:0000222_!_Illumina_sequencing_evidence&amp;diff=9830&amp;oldid=prev</id>
		<title>127.0.0.1: Add page for ECO:0000222_!_Illumina_sequencing_evidence</title>
		<link rel="alternate" type="text/html" href="https://microbialphenotypes.org/wiki/index.php?title=Category:ECO:0000222_!_Illumina_sequencing_evidence&amp;diff=9830&amp;oldid=prev"/>
		<updated>2013-03-05T20:52:12Z</updated>

		<summary type="html">&lt;p&gt;Add page for ECO:0000222_!_Illumina_sequencing_evidence&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;{{Autobox|1=&amp;#039;&amp;#039;&amp;#039;id:&amp;#039;&amp;#039;&amp;#039; ECO:0000222&amp;lt;br&amp;gt;&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;name:&amp;#039;&amp;#039;&amp;#039; Illumina sequencing evidence&amp;lt;br&amp;gt;&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;def:&amp;#039;&amp;#039;&amp;#039; &amp;quot;A high throughput nucleotide sequencing result derived by Illumina (Solexa) sequencing technology.&amp;quot; [ECO:MCC]&amp;lt;br&amp;gt;&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;synonym:&amp;#039;&amp;#039;&amp;#039; &amp;quot;Solexa sequencing result&amp;quot; RELATED [OBI:0000724]&amp;lt;br&amp;gt;&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;is_a:&amp;#039;&amp;#039;&amp;#039; [[:Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]][[Category:ECO:0000221 ! high throughput nucleotide sequencing assay evidence|ECO:0000221 ! high throughput nucleotide sequencing assay evidence]]&amp;lt;br&amp;gt;&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;comment:&amp;#039;&amp;#039;&amp;#039; In Illumina sequencing technology, adapters are ligated onto the ends of randomly fragmented DNA. After single-stranded fragments are bound to the inside surface of a flow cell channel, they undergo in-place bridge PCR amplification. To begin the sequencing cycle, labeled reversible terminators, primers and DNA polymerase are added, followed by laser excitation of the fluorescent label and high resolution scanning. After non-incorporated nucleotides are washed away and the dye is chemically removed, the next cycle repeats with four labeled reversible terminators, primers and DNA polymerase, again followed by imaging.&amp;lt;br&amp;gt;&lt;br /&gt;
}}&lt;br /&gt;
== Usage Notes ==&lt;br /&gt;
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==References ==&lt;br /&gt;
{{RefHelp}}&lt;br /&gt;
&amp;lt;references/&amp;gt;&lt;/div&gt;</summary>
		<author><name>127.0.0.1</name></author>
	</entry>
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