Category:ECO:0000118 ! subtractive hybridization evidence - Revision history

127.0.0.1: update ECO:0000118_!_subtractive_hybridization_evidence page

2018-06-16T07:01:21Z

update ECO:0000118_!_subtractive_hybridization_evidence page

127.0.0.1: update ECO:0000118_!_subtractive_hybridization_evidence page

2018-03-04T08:00:42Z

update ECO:0000118_!_subtractive_hybridization_evidence page

127.0.0.1: update ECO:0000118_!_subtractive_hybridization_evidence page

2017-12-04T08:00:59Z

update ECO:0000118_!_subtractive_hybridization_evidence page

127.0.0.1: Add page for ECO:0000118_!_subtractive_hybridization_evidence

2013-03-05T20:52:06Z

Add page for ECO:0000118_!_subtractive_hybridization_evidence

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{{Autobox|1='''id:''' ECO:0000118 
'''name:''' subtractive hybridization evidence 
'''def:''' "Used when an annotation is made based on results of a subtractive hybridization experiment that demonstrates expression of an RNA under one condition and not another." [TAIR:TED] 
'''synonym:''' "IEP: subtractive hybridization" RELATED [] 
'''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]] 
'''is_a:''' [[:Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]][[Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]] 
}}
== Usage Notes ==

==References ==
{{RefHelp}}
<references/>

@@ Line 4: / Line 4: @@
 '''synonym:''' "IEP: subtractive hybridization" RELATED []<br>
 '''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]]<br>
 '''comment:''' Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries.<br>
 }}

@@ Line 4: / Line 4: @@
 '''synonym:''' "IEP: subtractive hybridization" RELATED []<br>
 '''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]]<br>
 '''comment:''' Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries.<br>
 }}

@@ Line 1: / Line 1: @@
 {{Autobox|1='''id:''' ECO:0000118<br>
 '''name:''' subtractive hybridization evidence<br>
-'''def:''' "Used when an annotation is made based on results of a subtractive hybridization experiment that demonstrates expression of an RNA under one condition and not another." [TAIR:TED]<br>
+'''def:''' "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'." [PMID:11298187]<br>
 '''synonym:''' "IEP: subtractive hybridization" RELATED []<br>
 '''is_a:''' [[:Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]][[Category:ECO:0000009 ! transcript expression evidence|ECO:0000009 ! transcript expression evidence]]<br>
 '''is_a:''' [[:Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]][[Category:ECO:0000026 ! nucleic acid hybridization evidence|ECO:0000026 ! nucleic acid hybridization evidence]]<br>
 }}
 == Usage Notes ==