https://microbialphenotypes.org/wiki/index.php?action=history&feed=atom&title=Category%3AECO%3A0000112_%21_western_blot_evidence
Category:ECO:0000112 ! western blot evidence - Revision history
2026-09-28T03:54:14Z
Revision history for this page on the wiki
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https://microbialphenotypes.org/wiki/index.php?title=Category:ECO:0000112_!_western_blot_evidence&diff=21198&oldid=prev
127.0.0.1: update ECO:0000112_!_western_blot_evidence page
2016-08-09T17:01:24Z
<p>update ECO:0000112_!_western_blot_evidence page</p>
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 17:01, 9 August 2016</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Autobox|1='''id:''' ECO:0000112<br></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Autobox|1='''id:''' ECO:0000112<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''name:''' western blot evidence<br></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''name:''' western blot evidence<br></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>'''def:''' "A type of <del class="diffchange diffchange-inline">protein expression level </del>evidence where <del class="diffchange diffchange-inline">specific </del>proteins <del class="diffchange diffchange-inline">from a mixed sample </del>are <del class="diffchange diffchange-inline">detected </del>and <del class="diffchange diffchange-inline">their expression levels </del>are <del class="diffchange diffchange-inline">quantified using Western blotting </del>technique." [ECO:SW, PMID:23050259, TAIR:TED]<br></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>'''def:''' "A type of <ins class="diffchange diffchange-inline">gel electrophoresis </ins>evidence where proteins are <ins class="diffchange diffchange-inline">separated using gel electrophoresis </ins>and <ins class="diffchange diffchange-inline">then they </ins>are <ins class="diffchange diffchange-inline">blotted on a nitrocellulose or polyvinylidene difluoride (PVDF) membrane, later an antibody with a dye or an enzyme is added to the solution which is able to bind to its specific protein and this product is detected either by chemiluminescent detection technique or fluorescence imaging </ins>technique." [ECO:SW, PMID:23050259, TAIR:TED]<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''synonym:''' "IEP: protein levels (e.g. Western blots)" RELATED []<br></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''synonym:''' "IEP: protein levels (e.g. Western blots)" RELATED []<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''synonym:''' "protein immunoblot" RELATED []<br></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''synonym:''' "protein immunoblot" RELATED []<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''synonym:''' "Western blot expression analysis" RELATED []<br></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''synonym:''' "Western blot expression analysis" RELATED []<br></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>'''is_a:''' [[:Category:ECO:<del class="diffchange diffchange-inline">0000046 </del>! <del class="diffchange diffchange-inline">protein expression level </del>evidence|ECO:<del class="diffchange diffchange-inline">0000046 </del>! <del class="diffchange diffchange-inline">protein expression level </del>evidence]][[Category:ECO:<del class="diffchange diffchange-inline">0000046 </del>! <del class="diffchange diffchange-inline">protein expression level </del>evidence|ECO:<del class="diffchange diffchange-inline">0000046 </del>! <del class="diffchange diffchange-inline">protein expression level </del>evidence]]<br></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>'''is_a:''' [[:Category:ECO:<ins class="diffchange diffchange-inline">0000333 </ins>! <ins class="diffchange diffchange-inline">sodium dodecyl sulfate polyacrylamide gel electrophoresis </ins>evidence|ECO:<ins class="diffchange diffchange-inline">0000333 </ins>! <ins class="diffchange diffchange-inline">sodium dodecyl sulfate polyacrylamide gel electrophoresis </ins>evidence]][[Category:ECO:<ins class="diffchange diffchange-inline">0000333 </ins>! <ins class="diffchange diffchange-inline">sodium dodecyl sulfate polyacrylamide gel electrophoresis </ins>evidence|ECO:<ins class="diffchange diffchange-inline">0000333 </ins>! <ins class="diffchange diffchange-inline">sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence]]<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">'''is_a:''' [[:Category:ECO:0005593 ! immunodetection assay evidence|ECO:0005593 ! immunodetection assay evidence]][[Category:ECO:0005593 ! immunodetection assay evidence|ECO:0005593 ! immunodetection assay </ins>evidence]]<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''comment:''' Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies.<br></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>'''comment:''' Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
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127.0.0.1
https://microbialphenotypes.org/wiki/index.php?title=Category:ECO:0000112_!_western_blot_evidence&diff=18620&oldid=prev
127.0.0.1: Add page for ECO:0000112_!_western_blot_evidence
2015-12-18T08:03:07Z
<p>Add page for ECO:0000112_!_western_blot_evidence</p>
<p><b>New page</b></p><div>{{Autobox|1='''id:''' ECO:0000112<br><br />
'''name:''' western blot evidence<br><br />
'''def:''' "A type of protein expression level evidence where specific proteins from a mixed sample are detected and their expression levels are quantified using Western blotting technique." [ECO:SW, PMID:23050259, TAIR:TED]<br><br />
'''synonym:''' "IEP: protein levels (e.g. Western blots)" RELATED []<br><br />
'''synonym:''' "protein immunoblot" RELATED []<br><br />
'''synonym:''' "Western blot expression analysis" RELATED []<br><br />
'''is_a:''' [[:Category:ECO:0000046 ! protein expression level evidence|ECO:0000046 ! protein expression level evidence]][[Category:ECO:0000046 ! protein expression level evidence|ECO:0000046 ! protein expression level evidence]]<br><br />
'''comment:''' Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies.<br><br />
}}<br />
== Usage Notes ==<br />
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==References ==<br />
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127.0.0.1