Category:ECO:0000025 ! hybrid interaction evidence - Revision history

127.0.0.1: update ECO:0000025_!_hybrid_interaction_evidence page

2017-10-24T22:59:22Z

update ECO:0000025_!_hybrid_interaction_evidence page

← Older revision		Revision as of 22:59, 24 October 2017
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	'''name:''' hybrid interaction evidence<br>		'''name:''' hybrid interaction evidence<br>
	'''def:''' "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090]<br>		'''def:''' "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090]<br>
	'''xref:''' [~~http://psidev.sourceforge.net/mi/xml/doc/user/index.html PSI-~~MI~~:MI\~~:0090 "protein complementation assay"]<br>		'''xref:''' [ MI:0090 "protein complementation assay"]<br>
	'''is_a:''' [[:Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]][[Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]]<br>		'''is_a:''' [[:Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]][[Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]]<br>
	'''comment:''' Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.<br>		'''comment:''' Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.<br>

127.0.0.1: update ECO:0000025_!_hybrid_interaction_evidence page

2013-04-23T18:49:16Z

update ECO:0000025_!_hybrid_interaction_evidence page

← Older revision		Revision as of 18:49, 23 April 2013
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	'''name:''' hybrid interaction evidence<br>		'''name:''' hybrid interaction evidence<br>
	'''def:''' "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090]<br>		'''def:''' "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090]<br>
	'''xref:''' [ PSI-MI:MI\:0090 "protein complementation assay"]<br>		'''xref:''' [http://psidev.sourceforge.net/mi/xml/doc/user/index.html PSI-MI:MI\:0090 "protein complementation assay"]<br>
	'''is_a:''' [[:Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]][[Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]]<br>		'''is_a:''' [[:Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]][[Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]]<br>
	'''comment:''' Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.<br>		'''comment:''' Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.<br>

127.0.0.1: update ECO:0000025_!_hybrid_interaction_evidence page

2013-03-19T06:00:06Z

update ECO:0000025_!_hybrid_interaction_evidence page

← Older revision		Revision as of 06:00, 19 March 2013
Line 2:		Line 2:
	'''name:''' hybrid interaction evidence<br>		'''name:''' hybrid interaction evidence<br>
	'''def:''' "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090]<br>		'''def:''' "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090]<br>
	'''xref:''' [~~http://psidev.sourceforge.net/mi/xml/doc/user/index.html~~ PSI-MI:MI\:0090 "protein complementation assay"]<br>		'''xref:''' [ PSI-MI:MI\:0090 "protein complementation assay"]<br>
	'''is_a:''' [[:Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]][[Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]]<br>		'''is_a:''' [[:Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]][[Category:ECO:0000023 ! affinity evidence\|ECO:0000023 ! affinity evidence]]<br>
	'''comment:''' Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.<br>		'''comment:''' Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.<br>

127.0.0.1: Add page for ECO:0000025_!_hybrid_interaction_evidence

2013-03-05T20:51:56Z

Add page for ECO:0000025_!_hybrid_interaction_evidence

New page

{{Autobox|1='''id:''' ECO:0000025 
'''name:''' hybrid interaction evidence 
'''def:''' "A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090] 
'''xref:''' [http://psidev.sourceforge.net/mi/xml/doc/user/index.html PSI-MI:MI\:0090 "protein complementation assay"] 
'''is_a:''' [[:Category:ECO:0000023 ! affinity evidence|ECO:0000023 ! affinity evidence]][[Category:ECO:0000023 ! affinity evidence|ECO:0000023 ! affinity evidence]] 
'''comment:''' Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode. 
}}
== Usage Notes ==

==References ==
{{RefHelp}}
<references/>