View source for OMP ST:922 ! Escherichia coli K-12 MS1350

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==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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MS1350
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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F- 
lambda- 
e14-(?)
Rac-0(?)
uvrC34
hisG4(Oc)
rfbC1 
mgl-51
rpsL31(strR) 
kdgK51
xylA5
mtl-1
argE3(Oc) 
thiE1 
thyA 
galU51
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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*PMID:330498
*PMID:4567134
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent:[[OMP ST:777 ! Escherichia coli K-12 AB1884]]
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==
"Strain MS1350 was prepared by M. Silverman in M. Simon's laboratory from a K-12 strain, AB1884, obtained from J. Adler. 
The strain was made Lac+, Pro+, Gal+, Thr+,and Leu+ by conjugation with strain KL96, and then Trp- by bromodeoxyuridine auxotroph selection (4).
The galU marker was introduced by P1 transduction from W4597 by selecting for Trp+ Gal- recombinants. Thy- strains were obtained by trimethoprim treatment (19). The absence of suppressors was determined by the inability of phage T4 amber B22 to form plaques on this strain." <ref name='PMID:4567134'/>

AB1884 is Rac-0, and KL96 e14-. I haven't been able to determine from the literature whether MS1350 was checked for either Rac-0 or e14-, so in the genotype these alleles are marked with question marks.  [[User:Siegele|Siegele]] ([[User talk:Siegele|talk]]) 15:45, 20 July 2016 (CDT)

==Phenotype Annotation==
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!|Annotation ID!!not!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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OMP_AN:718
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OMP:0000005
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presence of cell motility
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Relative to: 

<hr>Genotype differences:<br>
Nothing to compare<br/>
<hr>Condition differences:<br>
Nothing to compare<br/>
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medium:Motility agar
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swim plate assay
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PMID:4567134
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OMP_AN:719
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Presence of flagella
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Relative to: 

<hr>Genotype differences:<br>
Nothing to compare<br/>
<hr>Condition differences:<br>
Nothing to compare<br/>
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ECO:0001098
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microscopy evidence
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PMID:4567134
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OMP_AN:737
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OMP:0007238
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sensitive to bacteriophage
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Relative to: 

<hr>Genotype differences:<br>
Nothing to compare<br/>
<hr>Condition differences:<br>
Nothing to compare<br/>
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PMID:4567134
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[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1541887 Taxonomy ID: 1541887 Bacteriophage Chi]
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==Additional Information==


==Links==


==References==
{{RefHelp}}<references/>