View source for OMP ST:74709 ! Escherichia coli K-12 CAG41254

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==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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Escherichia coli K-12 CAG41254
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=83333 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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F- Lambda- rph-1 Lamda(rpoHP3::lacZ) lacX74(del) nlpB(del)::Cm CmR
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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PMID:15968066
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent:OMP ST:74707 ! Escherichia coli K-12 CAG45114
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==
In order to confirm that the nlpB(del) deletion was responsible for the sensitivity to certain antimicrobial agents, a plasmid carrying the nlpB gene was inserted into this strain. The two transformants, CAG41534 and CAG41536, were tested and the EOP experiment was repeated using these transformants. After introducing the plasmids, the transformants displayed the wild-type phenotype.

==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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OMP_AN:27323
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NOT
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OMP:0007894
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altered resistance to a surfactant
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Relative to: OMP_AN:27317

<hr>Genotype differences:<br>
+nlpB(del) +CmR
<hr>Condition differences:<br>
no differences
|
*temperature:30C
*medium:LB broth
*medium:SDS (3.5%)
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ECO:0001165
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colony counting evidence used in manual assertion
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PMID:15968066
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[https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:8984 CHEBI:8984] SDS
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Table 5; EOP=0.8 
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OMP_AN:27324
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OMP:0007221
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abolished resistance to rifamycin
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Relative to: OMP_AN:27318

<hr>Genotype differences:<br>
+nlpB(del) +CmR
<hr>Condition differences:<br>
no differences
|
*temperature:30C
*medium:LB broth
*medium:rifampicin (5 mg/ml)
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ECO:0001165
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colony counting evidence used in manual assertion
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PMID:15968066
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[https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:28077 CHEBI:28077] rifampicin
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Table 5; EOP=4E-5 
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OMP_AN:27325
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NOT
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OMP:0007134
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altered resistance to an antimicrobial agent
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Relative to: OMP_AN:27319

<hr>Genotype differences:<br>
+nlpB(del) +CmR
<hr>Condition differences:<br>
no differences
|
*temperature:30C
*medium:LB broth
*medium:novobiocin (150 mg/ml)
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ECO:0001165
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colony counting evidence used in manual assertion
|
PMID:15968066
|
[https://www.ebi.ac.uk/chebi/searchId.do?chebiId=28368 CHEBI:28368] novobiocin
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Table 5; EOP=0.7 
|- 
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OMP_AN:27335
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NOT
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OMP:0007901
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altered resistance to bile acids
|
Relative to: OMP_AN:27332

<hr>Genotype differences:<br>
+nlpB(del) +CmR
<hr>Condition differences:<br>
no differences
|
*temperature:30C
*medium:MacConkey agar
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ECO:0001165
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colony counting evidence used in manual assertion
|
PMID:15968066
|

|
 
|- 
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OMP_AN:27345
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NOT
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OMP:0007001
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altered motility
|
Relative to: OMP_AN:27339

<hr>Genotype differences:<br>
+nlpB(del) +CmR
<hr>Condition differences:<br>
no differences
|
*temperature:30C
*medium:LB soft agar
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ECO:0007192
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motility assay evidence used in manual assertion
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PMID:15968066
|

|
 
|- 
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OMP_AN:27346
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NOT
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OMP:0007001
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altered motility
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Relative to: OMP_AN:27340

<hr>Genotype differences:<br>
+nlpB(del) +CmR
<hr>Condition differences:<br>
no differences
|
*temperature:37C
*medium:LB soft agar
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ECO:0007192
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motility assay evidence used in manual assertion
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PMID:15968066
|

|
 
|- 
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OMP_AN:27354
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NOT
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OMP:0007519
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altered population growth in high osmolarity
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Relative to: OMP_AN:27351

<hr>Genotype differences:<br>
+nlpB(del) +CmR
<hr>Condition differences:<br>
no differences
|
*temperature:30C
*medium:Low-osmolarity medium
*medium:NaCl
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ECO:0001258
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spectrophotometry evidence used in manual assertion
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PMID:15968066
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[https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:26710 CHEBI:26710] NaCl
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The low-osmolarity medium was supplemented with 0.1 M NaCl, 0.2 M NaCl, 0.3 M NaCl, 0.4 M NaCl 

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==Additional Information==


==Links==


==References==
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