View source for OMP ST:74274 ! Escherichia coli K-12 JW5377

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==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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Escherichia coli K-12 JW5377
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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JW5377-1
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 yfcE782(del)::FRT-kan-FRT
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=109632 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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[http://cgsc2.biology.yale.edu/Strain.php?ID=109632 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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OMP_AN:36057
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OMP:0007075
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decreased growth factor requirement
|
Relative to: OMP_AN:27604

<hr>Genotype differences:<br>
+lacZ4787(del)( +yfcE782(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:17754) Glycerol
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*chemical concentration= 0.4%
*normalized fitness= -3.86 
|- 
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OMP_AN:36058
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OMP:0007070
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decreased organic carbon source utilization
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Relative to: OMP_AN:27614

<hr>Genotype differences:<br>
+lacZ4787(del)( +yfcE782(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:M9
*medium:Glycerol
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ECO:0007005
|
high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:17754) Glycerol
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*chemical concentration= 0.4%
*normalized fitness= -3.86 
|- 
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OMP_AN:36059
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OMP:0007376
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increased resistance to an alkylating agent
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Relative to: OMP_AN:27502

<hr>Genotype differences:<br>
+lacZ4787(del)( +yfcE782(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:methyl methanesulfonate (MMS)
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:25255) methyl methanesulfonate (MMS)
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*chemical concentration= 0.05%
*normalized fitness= 3.37 
|- 
|
OMP_AN:36060
|

|
OMP:0006045
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increased resistance to beta-lactam compound
|
Relative to: OMP_AN:27532

<hr>Genotype differences:<br>
+lacZ4787(del)( +yfcE782(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:LB agar
*medium:Aztreonam
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ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:161680) Aztreonam
|

*chemical concentration=  0.04 ug/ml
*normalized fitness= 3.85 
|- 
|
OMP_AN:36061
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27598

<hr>Genotype differences:<br>
+lacZ4787(del)( +yfcE782(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -3.71 
|- 
|
OMP_AN:36062
|

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OMP:0007886
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decreased resistance to a metal
|
Relative to: OMP_AN:27608

<hr>Genotype differences:<br>
+lacZ4787(del)( +yfcE782(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron excess-FeSO4 heptahydride
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron excess-FeSO4 heptahydride
|

*chemical concentration= 1mM (MOPS; normal 100uM)
*normalized fitness= -3.71 
|- 
|
OMP_AN:36063
|

|
OMP:0007075
|
decreased growth factor requirement
|
Relative to: OMP_AN:27599

<hr>Genotype differences:<br>
+lacZ4787(del)( +yfcE782(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
|
PMID:21185072
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towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -4.18 
|- 
|
OMP_AN:36064
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OMP:0007937
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decreased resistance to nutrient limitation
|
Relative to: OMP_AN:27609

<hr>Genotype differences:<br>
+lacZ4787(del)( +yfcE782(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
|

*temperature:37C
*medium:MOPS
*medium:Iron starvation-FeSO4·7H2O
|
ECO:0007005
|
high throughput direct assay evidence used in manual assertion
|
PMID:21185072
|
towards some (CHEBI:75836) Iron starvation-FeSO4·7H2O
|

*chemical concentration= 2uM (MOPS; normal 100uM)
*normalized fitness= -4.18 

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==Additional Information==


==Links==


==References==
{{RefHelp}}<references/>