View source for OMP ST:74254 ! Escherichia coli K-12 JW5349

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==Strain Summary==
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!align=left align='left' bgcolor='#CCCCFF' |Strain Name
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Escherichia coli K-12 JW5349
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!align=left align='left' bgcolor='#CCCCFF' |Synonyms
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JW5349-1
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!align=left align='left' bgcolor='#CCCCFF' |Taxon Information
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*Pangenome: Escherichia coli
*Subspecies and/or strain: K-12
*NCBI Taxonomy ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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!align=left align='left' bgcolor='#CCCCFF' |Genotype
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DE(araD-araB)567 DE(rhaD-rhaB)568 F- Lambda- hsdR514 lacZ4787(del)(::rrnB-3) rph-1 yehL744(del)::FRT-kan-FRT
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!align=left align='left' bgcolor='#CCCCFF' |Strain Reference
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*PMID:16738554
*[http://cgsc2.biology.yale.edu/Strain.php?ID=109614 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Strain availability
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[http://cgsc2.biology.yale.edu/Strain.php?ID=109614 Coli Genetic Stock Center]
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!align=left align='left' bgcolor='#CCCCFF' |Ancestry
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parent: [[OMP ST:800 ! Escherichia coli K-12 BW25113]]
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!align=left align='left' bgcolor='#CCCCFF' |Annotated phenotypes
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==Notes==


==Phenotype Annotation==
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!|Annotation ID!!Qualifier!!OMP ID!!OMP term name!!Relative phenotype information!!Experimental condition!!ECO ID!!ECO term name!!Reference!!Annotation Extension!!Notes
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OMP_AN:36030
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OMP:0007896
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decreased resistance to a surfactant
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Relative to: OMP_AN:27581

<hr>Genotype differences:<br>
+lacZ4787(del)( +yehL744(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
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*temperature:37C
*medium:LB agar
*medium:Benzalkonium chloride
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:3020) Benzalkonium chloride
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*chemical concentration=  25 ug/ml
*normalized fitness= -3.88 
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OMP_AN:36031
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OMP:0007879
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decreased resistance to an antimicrobial agent
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Relative to: OMP_AN:27590

<hr>Genotype differences:<br>
+lacZ4787(del)( +yehL744(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
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*temperature:37C
*medium:LB agar
*medium:Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CID:6093162 + CHEBI:4170) Fosfomycin (CID:6093162) +Glucose-6-phosphate (CHEBI:4170)
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*chemical concentration= 0.05 ug/ml (in 50 mg/ml G6P)
*normalized fitness= -3.59 
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OMP_AN:36032
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OMP:0006058
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increased resistance to aminoglycoside
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Relative to: OMP_AN:27528

<hr>Genotype differences:<br>
+lacZ4787(del)( +yehL744(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
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*temperature:37C
*medium:LB agar
*medium:Streptomycin sulfate
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (CHEBI:32158) Streptomycin sulfate
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*chemical concentration= 0.05 ug/ml
*normalized fitness= 3.39 
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OMP_AN:36033
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OMP:0007010
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decreased population growth at alkaline pH
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Relative to: OMP_AN:27623

<hr>Genotype differences:<br>
+lacZ4787(del)( +yehL744(del)  -lacZ4787(del)
<hr>Condition differences:<br>
no differences
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*temperature:37C
*medium:LB agar
*medium:TAPS
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ECO:0007005
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high throughput direct assay evidence used in manual assertion
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PMID:21185072
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towards some (**SID:24900259) TAPS
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*chemical concentration=  9.5 
*normalized fitness= -3.56 

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==Additional Information==


==Links==


==References==
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